{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T18:31:14Z","timestamp":1786559474888,"version":"build-2736575974"},"reference-count":76,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2023,1,10]],"date-time":"2023-01-10T00:00:00Z","timestamp":1673308800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Thyroid is a glandular tissue in the human body in which the function can be severely affected by endocrine disrupting chemicals (EDCs). Current in vitro assays to test endocrine disruption by chemical compounds are largely based on 2D\u00a0thyroid cell cultures, which often fail to precisely evaluate the safety of these compounds. New and more advanced 3D\u00a0cell culture systems are urgently needed to better recapitulate the thyroid follicular architecture and functions and help to improve the predictive power of such assays. Herein, the development of a thyroid organoid\u2010on\u2010a\u2010chip (OoC) device using polymeric membranous carriers is described. Mouse embryonic stem cell derived thyroid follicles are incorporated in a microfluidic chip for a 4 day experiment at a flow rate of 12\u00a0\u00b5L min<jats:sup>\u22121<\/jats:sup>. A reversible seal provides a leak\u2010tight sealing while enabling quick and easy loading\/unloading of thyroid follicles. The OoC model shows a high degree of functionality, where organoids retain expression of key thyroid genes and a typical follicular structure. Finally, transcriptional changes following benzo[<jats:italic>k<\/jats:italic>]fluoranthene exposure in the OoC device demonstrate activation of the xenobiotic aryl hydrocarbon receptor pathway. Altogether, this OoC system is a physiologically relevant thyroid model, which will represent a valuable tool to test potential EDCs.<\/jats:p>","DOI":"10.1002\/adhm.202201555","type":"journal-article","created":{"date-parts":[[2022,12,22]],"date-time":"2022-12-22T08:01:36Z","timestamp":1671696096000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["Thyroid\u2010on\u2010a\u2010Chip: An Organoid Platform for In Vitro Assessment of Endocrine Disruption"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2664-5889","authenticated-orcid":false,"given":"Daniel J.","family":"Carvalho","sequence":"first","affiliation":[{"name":"Department of Instructive Biomaterials Engineering MERLN Institute for Technology\u2010Inspired Regenerative Medicine Maastricht University  Maastricht 6229 ER The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna M.","family":"Kip","sequence":"additional","affiliation":[{"name":"Department of Complex Tissue Regeneration MERLN Institute for Technology\u2010Inspired Regenerative Medicine Maastricht University  Maastricht 6229 ER The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00edrian","family":"Romitti","sequence":"additional","affiliation":[{"name":"Institute of Interdisciplinary Research in Molecular Human Biology (IRIBHM) Universit\u00e9 Libre de Bruxelles  808 route de Lennik Brussels 1070 Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Nazzari","sequence":"additional","affiliation":[{"name":"Department of Toxicogenomics GROW School for Oncology and Developmental Biology Maastricht University  Maastricht 6229 ER The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Tegel","sequence":"additional","affiliation":[{"name":"PreSens Precision Sensing GmbH  Am Biopark 11 93053 Regensburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias","family":"Stich","sequence":"additional","affiliation":[{"name":"PreSens Precision Sensing GmbH  Am Biopark 11 93053 Regensburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Krause","sequence":"additional","affiliation":[{"name":"PreSens Precision Sensing GmbH  Am Biopark 11 93053 Regensburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florian","family":"Caiment","sequence":"additional","affiliation":[{"name":"Department of Toxicogenomics GROW School for Oncology and Developmental Biology Maastricht University  Maastricht 6229 ER The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabine","family":"Costagliola","sequence":"additional","affiliation":[{"name":"Institute of Interdisciplinary Research in Molecular Human Biology (IRIBHM) Universit\u00e9 Libre de Bruxelles  808 route de Lennik Brussels 1070 Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorenzo","family":"Moroni","sequence":"additional","affiliation":[{"name":"Department of Complex Tissue Regeneration MERLN Institute for Technology\u2010Inspired Regenerative Medicine Maastricht University  Maastricht 6229 ER The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1354-0167","authenticated-orcid":false,"given":"Stefan","family":"Giselbrecht","sequence":"additional","affiliation":[{"name":"Department of Instructive Biomaterials Engineering MERLN Institute for Technology\u2010Inspired Regenerative Medicine Maastricht University  Maastricht 6229 ER The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,1,10]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrendo.2018.18"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/10937404.2011.578556"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2011.09.005"},{"key":"e_1_2_9_4_1","volume-title":"Endocrinology: Adult & Pediatric","author":"Jameson J. 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