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In particular, they represent promising drug delivery systems. Nanogel synthesis by oxidative condensation of polyglycidol prepolymers, that are modified with thiol groups, results in crosslinking by disulfide bonds. Hereby, biomolecules like the antidiabetic peptide RS1\u2010reg, derived from the regulatory protein RS1 of the Na<jats:sup>+<\/jats:sup>\u2010D\u2010glucose cotransporter SGLT1, can be covalently bound by cysteine residues to the nanogel in a hydrophilic, stabilizing environment. After oral uptake, the acid\u2010stable nanogels protect their loading during gastric passage from proteolytic degradation. Under alkaline conditions in small intestine the nanohydrogels become mucoadhesive, pass the intestinal mucosa and are taken up into small intestinal enterocytes by endocytosis. Using Caco\u20102 cells as a\u00a0model for small intestinal enterocytes, by confocal laser scanning microscopy and structured illumination microscopy, the colocalization of fluorescent\u2010labeled RS1\u2010reg with markers of endosomes, lysosomes, and trans\u2010Golgi\u2010network after uptake with polyglycidol\u2010based nanogels formed by precipitation polymerization is demonstrated. This indicates that RS1\u2010reg follows the endosomal pathway. In the following, the design of bespoken nanohydrogels for specific targeting of RS1\u2010reg to its site of action at the trans\u2010Golgi network is described that might also represent a way of targeted transport for other drugs to their targets at the Golgi apparatus.<\/jats:p>","DOI":"10.1002\/adhm.202201794","type":"journal-article","created":{"date-parts":[[2023,2,4]],"date-time":"2023-02-04T22:29:40Z","timestamp":1675549780000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Design of Nanohydrogels for Targeted Intracellular Drug Transport to the Trans\u2010Golgi Network"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2897-5761","authenticated-orcid":false,"given":"Thorsten","family":"Keller","sequence":"first","affiliation":[{"name":"Department for Functional Materials in Medicine and Dentistry, Institute of Functional Materials and Biofabrication University of W\u00fcrzburg  Pleicherwall 2 97070 W\u00fcrzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nora","family":"Trinks","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Biophysics University of W\u00fcrzburg  Am Hubland 97074 W\u00fcrzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica","family":"Brand","sequence":"additional","affiliation":[{"name":"Department for Functional Materials in Medicine and Dentistry, Institute of Functional Materials and Biofabrication University of W\u00fcrzburg  Pleicherwall 2 97070 W\u00fcrzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steffen","family":"Trippmacher","sequence":"additional","affiliation":[{"name":"Physical Chemistry II University of Bayreuth  Universit\u00e4tsstr. 30 95440 Bayreuth Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philipp","family":"Stahlhut","sequence":"additional","affiliation":[{"name":"Department for Functional Materials in Medicine and Dentistry, Institute of Functional Materials and Biofabrication University of W\u00fcrzburg  Pleicherwall 2 97070 W\u00fcrzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krystyna","family":"Albrecht","sequence":"additional","affiliation":[{"name":"Department for Functional Materials in Medicine and Dentistry, Institute of Functional Materials and Biofabrication University of W\u00fcrzburg  Pleicherwall 2 97070 W\u00fcrzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5870-6693","authenticated-orcid":false,"given":"Georg","family":"Papastavrou","sequence":"additional","affiliation":[{"name":"Physical Chemistry II University of Bayreuth  Universit\u00e4tsstr. 30 95440 Bayreuth Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5118-7088","authenticated-orcid":false,"given":"Hermann","family":"Koepsell","sequence":"additional","affiliation":[{"name":"Institute of Anatomy and Cell Biology University of W\u00fcrzburg  Koellikerstra\u00dfe 6 97070 W\u00fcrzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1692-3219","authenticated-orcid":false,"given":"Markus","family":"Sauer","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Biophysics University of W\u00fcrzburg  Am Hubland 97074 W\u00fcrzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3167-8466","authenticated-orcid":false,"given":"J\u00fcrgen","family":"Groll","sequence":"additional","affiliation":[{"name":"Department for Functional Materials in Medicine and Dentistry, Institute of Functional Materials and Biofabrication University of W\u00fcrzburg  Pleicherwall 2 97070 W\u00fcrzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,2,24]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0939-6411(00)00090-4"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0169-409X(93)90027-2"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1015887213431"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/ma2020577"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2019.06.005"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00396-008-1949-0"},{"key":"e_1_2_9_7_1","first-page":"3320","volume":"8","author":"Gao D.","year":"2008","journal-title":"Adv. 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