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Target\u2010catalyzed toehold\u2010mediated strand displacement (TMSD) has received increasing attention as an enzyme\u2010free molecular tool for DNA detection. However, the application of TMSD to miRNA targets is challenging because relatively weak DNA\/RNA hybridization leads to failure in the subtle kinetic control of multiple hybridization steps. Here, a simple method is presented for miRNA assay based on the one\u2010pot self\u2010assembly of Y\u2010shaped DNAs with streptavidin via an miRNA\u2010catalyzed TMSD cascade reaction. A single miRNA catalyzes the opening cycle of DNA hairpin loops to generate multiple Y\u2010shaped DNAs carrying biotin and a quencher at the end of their arms. Introducing a single base\u2010pair mismatch near the toehold facilitates RNA\u2010triggered strand displacement while barely disturbing nonspecific reactions. The Y\u2010shaped DNAs are self\u2010assembled with fluorescently labeled streptavidin (sAv), which produces nanoscale DNA\u2010sAv nanogel particles mediating efficient F\u00f6rster resonance energy transfer in their 3D network. The enhancing effect dramatically reduces the detection limit from the nanomolar level to the picomolar level. This work proves that TMSD\u2010based DNA nanogel with a base\u2010pair mismatch incorporated to a hairpin structure is a promising approach towards sensitive and accurate miRNA assay.<\/jats:p>","DOI":"10.1002\/adhm.202202076","type":"journal-article","created":{"date-parts":[[2022,12,29]],"date-time":"2022-12-29T04:59:36Z","timestamp":1672289976000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Target\u2010Catalyzed Self\u2010Assembly of DNA\u2010Streptavidin Nanogel for Enzyme\u2010Free miRNA Assay"],"prefix":"10.1002","volume":"12","author":[{"given":"Jae Chul","family":"Park","sequence":"first","affiliation":[{"name":"Department of Materials Science and Engineering Korea Advanced Institute of Science and Technology  291 Daehak\u2010ro, Yuseong\u2010gu Daejeon 34141 Republic of 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