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This work describes a light\u2010based technique for rapidly annealing microgels across a range of diameters. Utilizing 8\u2010arm poly(ethylene)\u00a0glycol\u2010vinyl sulfone, the number of arms available for crosslinking, functionalization, and annealing is stoichiometrically controlled. Small and large microgels are fabricated to explore how microgel diameter impacts void space and the role of porosity on cell spreading, cell aggregation, and macrophage polarization. Mesenchymal stromal cells spread rapidly in both formulations, yet the smaller microgels permit a higher cell density. When seeded with macrophages, the smaller microgels promote an M1 phenotype, while larger microgels promote an M2 phenotype. As another application, the inherent porosity of annealed microgels is leveraged to induce cell aggregation. Finally, the microgels are implanted to examine how different size microgels influence endogenous cell invasion and macrophage polarization. The use of ultraviolet light allows for microgels to be noninvasively injected into a desired mold or wound defect before annealing, and microgels of different properties combined to create a heterogeneous scaffold. This approach is clinically relevant given its tunability and fast annealing time.<\/jats:p>","DOI":"10.1002\/adhm.202202239","type":"journal-article","created":{"date-parts":[[2023,1,31]],"date-time":"2023-01-31T14:19:40Z","timestamp":1675174780000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":58,"title":["Multisized Photoannealable Microgels Regulate Cell Spreading, Aggregation, and Macrophage Phenotype through Microporous Void Space"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1824-1780","authenticated-orcid":false,"given":"Jeremy M.","family":"Lowen","sequence":"first","affiliation":[{"name":"Department of Orthopaedic Surgery UC Davis Health  Sacramento CA 95817 USA"},{"name":"Department of Biomedical Engineering University of California  Davis CA 95616 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7360-8736","authenticated-orcid":false,"given":"Gabriella C.","family":"Bond","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery UC Davis Health  Sacramento CA 95817 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1207-4624","authenticated-orcid":false,"given":"Katherine H.","family":"Griffin","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery UC Davis Health  Sacramento CA 95817 USA"},{"name":"School of Veterinary Medicine University of California  Davis CA 95616 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2397-5359","authenticated-orcid":false,"given":"Nathan K.","family":"Shimamoto","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery UC Davis Health  Sacramento CA 95817 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1009-6432","authenticated-orcid":false,"given":"Victoria L.","family":"Thai","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery UC Davis Health  Sacramento CA 95817 USA"},{"name":"Department of Biomedical Engineering University of California  Davis CA 95616 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1673-3335","authenticated-orcid":false,"given":"J. 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