{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:19:24Z","timestamp":1786976364537,"version":"build-2736575974"},"reference-count":47,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2022,12,18]],"date-time":"2022-12-18T00:00:00Z","timestamp":1671321600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82072378"],"award-info":[{"award-number":["82072378"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81601856"],"award-info":[{"award-number":["81601856"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Non\u2010small cell lung cancer (NSCLC) is the most common type of lung cancer with substantial morbidity and mortality. Herein, a new signal\u2010on electrochemiluminescence (ECL) immunosensor based on multiple amplification strategies is constructed for ultrasensitive detection of cytokeratin 19 fragment antigen 21\u20101 (CYFRA21\u20101) biomarker related to NSCLC. Polyethyleneimine (PEI) functionalized MXene is decorated with NiMn layer double hydroxide (NiMn LDH) to form MXene\u2010PEI\u2010NiMn LDH composite. Specially, the La\u2010MOF@ZIF\u201067 bimetallic organic framework (named as LZBM) and MXene\u2010PEI\u2010NiMn LDH both served as coreaction accelerators to improve the ECL emission of the luminol\u2010H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    system. To be specific, Au nanoparticles (AuNPs) coated MXene\u2010PEI\u2010NiMn LDH is applied to immobilize primary CYFRA21\u20101 antibody (Ab\n                    <jats:sub>1<\/jats:sub>\n                    ), while AuNPs decorated LZBM was used for the loading of luminol and secondary CYFRA21\u20101 antibody (Ab\n                    <jats:sub>2<\/jats:sub>\n                    ) to form tracer label. Therefore, the ECL signal of the sandwich\u2010type immunosensor is significantly enhanced due to the high loading capability for luminol and the synergistic catalytic ability for the decomposition of H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    into reactive oxygen species (ROS). Under the optimal experimental conditions, the ECL immunosensor exhibited good analytical performances for CYFRA21\u20101 detection with a wide linear range (100 fg mL\n                    <jats:sup>\u22121<\/jats:sup>\n                    \u2212100 ng mL\n                    <jats:sup>\u22121<\/jats:sup>\n                    ) and a low limit of detection (85.20 fg mL\n                    <jats:sup>\u22121<\/jats:sup>\n                    ), providing a promising method for early diagnosis of NSCLC.\n                  <\/jats:p>","DOI":"10.1002\/adhm.202202287","type":"journal-article","created":{"date-parts":[[2022,12,9]],"date-time":"2022-12-09T15:09:35Z","timestamp":1670598575000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["A Novel Signal\u2010On Electrochemiluminescence Immunosensor for the Detection of NSCLC Antigen Biomarker Based on New Co\u2010Reaction Accelerators"],"prefix":"10.1002","volume":"12","author":[{"given":"Lei","family":"Guo","sequence":"first","affiliation":[{"name":"Chongqing Research Center for Pharmaceutical Engineering College of Pharmacy Chongqing Medical University  Chongqing 400016 P. 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