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Thus, there is a demand for more convenient ways to obtain samples. Modern analytical techniques enable monitoring of multiple bioanalytes in smaller samples, opening possibilities for new matrices, and microsampling technologies to be adopted. Interstitial fluid (ISF) is an attractive alternative matrix that shows good correlation with plasma concentration dynamics for several analytes and can be sampled in a minimally invasive and painless manner from the skin at the point\u2010of\u2010care. However, there is currently a lack of sampling devices compatible with clinical translation. Here, to tackle state\u2010of\u2010the\u2010art limitations, a cost\u2010effective and compact single\u2010microneedle\u2010based device designed to painlessly collect precisely 1.1\u00a0\u00b5L of dermal ISF within minutes is presented. The fluid is volume\u2010metered, dried, and stably stored into analytical\u2010grade paper within the microfluidic device. The obtained sample can be mailed to a laboratory, quantitatively analyzed, and provide molecular insights comparable to blood testing. In a human study, the possibility to monitor various classes of molecular analytes is demonstrated in ISF microsamples, including caffeine, hundreds of proteins, and SARS\u2010CoV\u20102 antibodies, some being detected in ISF for the first time.<\/jats:p>","DOI":"10.1002\/adhm.202202564","type":"journal-article","created":{"date-parts":[[2023,2,7]],"date-time":"2023-02-07T06:54:27Z","timestamp":1675752867000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":57,"title":["Microneedle Patch for Painless Intradermal Collection of Interstitial Fluid Enabling Multianalyte Measurement of Small Molecules, SARS\u2010CoV\u20102\u00a0Antibodies, and Protein Profiling"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3549-0228","authenticated-orcid":false,"given":"Federico","family":"Ribet","sequence":"first","affiliation":[{"name":"Division of Micro and Nanosystems School of Electrical Engineering and 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