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The rarity and heterogeneity of MelCTCs have greatly limited the development of MelCTCs capture methods, especially those based on immune\/aptamer\u2010affinity. Herein, an extracellular vesicles\u2010camouflaged strategy is designed to functionalize the magnetic nanoparticles (Fe<jats:sub>3<\/jats:sub>O<jats:sub>4<\/jats:sub>) and to generate magnetic vesicles (Fe<jats:sub>3<\/jats:sub>O<jats:sub>4<\/jats:sub>@lip\/ev) with excellent antifouling and active tumor cell targeting properties. Combined with the bioorthogonal click chemistry, the engineered magnetic vesicles with dibenzocyclooctyne can be widely used to target and separate all the metabolically labeled CTCs with varied phenotypes, organ origin, and even the biological species. The capture efficiency exceeded 80% with an extremely low detection limitation of ten cells. Most importantly, the strategy proposed can be directly applied to enrich MelCTCs from 0.5\u00a0mL blood samples of melanoma\u2010bearing mice, with a greatly minimized residue of white blood cells (only 21\u2013568) while ignoring the fluctuations of MelCTC phenotype.<\/jats:p>","DOI":"10.1002\/adhm.202202825","type":"journal-article","created":{"date-parts":[[2023,2,11]],"date-time":"2023-02-11T15:59:36Z","timestamp":1676131176000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Hybrid Extracellular Vesicles\u2010Liposomes Camouflaged Magnetic Vesicles Cooperating with Bioorthogonal Click Chemistry for High\u2010Efficient Melanoma Circulating Tumor Cells Enrichment"],"prefix":"10.1002","volume":"12","author":[{"given":"Ke","family":"Kang","sequence":"first","affiliation":[{"name":"National Engineering Research Center for Biomaterials Sichuan University  Chengdu 610064 P. R. 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