{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:19:07Z","timestamp":1786976347597,"version":"build-2736575974"},"reference-count":72,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2023,2,17]],"date-time":"2023-02-17T00:00:00Z","timestamp":1676592000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2023,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Quantitative polymerase chain reaction (qPCR) is considered the gold standard for pathogen detection. However, improvement is still required, especially regarding the possibilities of decentralization. Apart from other reasons, infectious diseases demand on\u2010site analysis to avoid pathogen spreading and increase treatment efficacy. In this paper, the detection of SARS\u2010CoV\u20102 is carried out by reverse transcription loop\u2010mediated isothermal amplification, which has the advantage of requiring simple equipment, easily adaptable to decentralized analysis. It is proposed, for the first time, the use of palladium nanoclusters (PdNCs) as indicators of the amplification reaction at end point. The pH of the medium decreases during the reaction and, in turn, a variation in the catalytic activity of PdNCs on the oxygen reduction reaction (ORR) can be electrochemically observed. For the detection, flexible and small\u2010size screen\u2010printed electrodes can be premodified with PdNCs, which together with the use of a simple and small electrochemical equipment would greatly facilitates their integration in field\u2010deployable devices. This would allow a faster detection of SARS\u2010CoV\u20102 as well as of other future microbial threats after an easy adaptation.<\/jats:p>","DOI":"10.1002\/adhm.202202972","type":"journal-article","created":{"date-parts":[[2023,1,30]],"date-time":"2023-01-30T07:23:15Z","timestamp":1675063395000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Electrocatalytic Palladium Nanoclusters as Versatile Indicators of Bioassays: Rapid Electroanalytical Detection of SARS\u2010CoV\u20102 by Reverse Transcription Loop\u2010Mediated Isothermal Amplification"],"prefix":"10.1002","volume":"12","author":[{"given":"Alejandro","family":"Rodr\u00edguez\u2010Penedo","sequence":"first","affiliation":[{"name":"Deparment of Physical and Analytical Chemistry University of Oviedo  Oviedo 33006 Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pablo","family":"Riobo\u00f3\u2010Legaspi","sequence":"additional","affiliation":[{"name":"Deparment of Physical and Analytical Chemistry University of Oviedo  Oviedo 33006 Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Gonz\u00e1lez\u2010L\u00f3pez","sequence":"additional","affiliation":[{"name":"Deparment of Physical and Analytical Chemistry University of Oviedo  Oviedo 33006 Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Lores\u2010Pad\u00edn","sequence":"additional","affiliation":[{"name":"Deparment of Physical and Analytical Chemistry University of Oviedo  Oviedo 33006 Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosario","family":"Pereiro","sequence":"additional","affiliation":[{"name":"Deparment of Physical and Analytical Chemistry University of Oviedo  Oviedo 33006 Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda del Mar","family":"Garc\u00eda\u2010Su\u00e1rez","sequence":"additional","affiliation":[{"name":"School of Engineering and Technology International University of La Rioja  Logro\u00f1o 26006 Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Dolores","family":"Cima\u2010Cabal","sequence":"additional","affiliation":[{"name":"School of Engineering and Technology International University of La Rioja  Logro\u00f1o 26006 Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Estefan\u00eda","family":"Costa\u2010Rama","sequence":"additional","affiliation":[{"name":"Deparment of Physical and Analytical Chemistry University of Oviedo  Oviedo 33006 Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beatriz","family":"Fern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Deparment of Physical and Analytical Chemistry University of Oviedo  Oviedo 33006 Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3782-5025","authenticated-orcid":false,"given":"M. Teresa","family":"Fern\u00e1ndez\u2010Abedul","sequence":"additional","affiliation":[{"name":"Deparment of Physical and Analytical Chemistry University of Oviedo  Oviedo 33006 Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,2,17]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.6b04630"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S267212"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamanetworkopen.2021.26931"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eclinm.2021.100924"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2020.104529"},{"key":"e_1_2_8_6_1","unstructured":"W. H. Organization Laboratory testing for coronavirus disease (COVID\u201019) in suspected human cases: interim guidance 19 March 2020.https:\/\/apps.who.int\/iris\/handle\/10665\/331501(accessed: October 2022)."},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/acssensors.7b00299"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2666-5247(20)30121-X"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/biology9080182"},{"key":"e_1_2_8_10_1","first-page":"166","volume":"17","author":"Dhama K.","year":"2014","journal-title":"Pak. J. Biol. Sci."},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1261\/rna.076232.120"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/jam.13647"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.2144\/000113072"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.2144\/000114253"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-27076-z"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1812296116"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/acssensors.7b00825"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2017.10.019"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1080\/08830185.2021.1872566"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/celc.201600758"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jece.2022.107488"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2020.129118"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1039\/C8RA04050H"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physchem.58.032806.104546"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1039\/C3CC47512C"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1039\/C4NR01130A"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/nn4053217"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.9b11079"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11244-018-0884-7"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/ma11020191"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2020.121489"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-1097.2008.00434.x"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trechm.2020.04.011"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/ejic.200800569"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tetlet.2008.06.089"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsaem.8b01783"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1039\/D0TA06942F"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1039\/D0NJ03645E"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/celc.201600053"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1039\/D0EE03915B"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.3390\/catal8020065"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.susc.2008.05.036"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1002\/ange.200462335"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1021\/jp047349j"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1088\/1468-6996\/12\/4\/044606"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10800-018-1258-3"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep35252"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2009.07.002"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/elan.201900119"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1002\/elan.201700845"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2021.113926"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.enzmictec.2021.109855"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1002\/elan.201400421"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijhydene.2021.05.096"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1039\/C6CY01709F"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1039\/b108260b"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1186\/1556-276X-7-277"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/0013-4686(87)87021-4"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-018-0118-9"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.1111\/1751-7915.13586"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.2c02127"},{"key":"e_1_2_8_62_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.abc7075"},{"key":"e_1_2_8_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2017.10.022"},{"key":"e_1_2_8_64_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-018-1552-2"},{"key":"e_1_2_8_65_1","unstructured":"New England Biolabs https:\/\/international.neb.com\/faqs\/2021\/07\/27\/amplification\u2010occurred\u2010in\u2010my\u2010ntc\u2010samples\u2010following\u2010isothermal\u2010incubation\u2010what\u2010happened(accessed: November 2022)."},{"key":"e_1_2_8_66_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.0c05059"},{"key":"e_1_2_8_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2020.112437"},{"key":"e_1_2_8_68_1","doi-asserted-by":"publisher","DOI":"10.3390\/bios9030089"},{"key":"e_1_2_8_69_1","unstructured":"New England Biolabs https:\/\/international.neb.com\/faqs\/2021\/07\/27\/what\u2010are\u2010the\u2010advantages\u2010of\u2010using\u2010standard\u2010lamp\u2010neb\u2010e1700\u2010m1708\u2010e1708\u2010over\u2010ph\u2010based\u2010colorimetric\u2010lamp\u2010neb\u2010m1800\u2010m1804(accessed: November 2022)."},{"key":"e_1_2_8_70_1","unstructured":"B.Gates TED Conferences 2022.https:\/\/www.ted.com\/talks\/bill_gates_we_can_make_covid_19_the_last_pandemic(accessed: 16 November 2022)."},{"key":"e_1_2_8_71_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2021.05.053"},{"key":"e_1_2_8_72_1","author":"Ranoa D. R. E.","year":"2020","journal-title":"bioRxiv"}],"container-title":["Advanced Healthcare Materials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adhm.202202972","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/adhm.202202972","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adhm.202202972","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T13:21:45Z","timestamp":1759756905000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/adhm.202202972"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,2,17]]},"references-count":72,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2023,5]]}},"alternative-id":["10.1002\/adhm.202202972"],"URL":"https:\/\/doi.org\/10.1002\/adhm.202202972","archive":["Portico"],"relation":{},"ISSN":["2192-2640","2192-2659"],"issn-type":[{"value":"2192-2640","type":"print"},{"value":"2192-2659","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,2,17]]},"assertion":[{"value":"2022-11-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-02-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2202972"}}