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In this study, polyampholyte\u2010based (succinylated <jats:italic>\u03b5<\/jats:italic>\u2010poly\u2010<jats:sc>l<\/jats:sc>\u2010lysine) hydrogels containing polyvinyl alcohol and polyethylene glycol polymer matrices to stabilize proteins are developed. These hydrogels facilitated the loading and release of therapeutic amounts of proteins and withstood thermal and freezing stress (15 freeze\u2013thaw cycles and temperatures of \u221280 \u00b0C and 37 \u00b0C), without resulting in protein denaturation and aggregation. To the best of our knowledge, this strategy has not been applied to the design of hydrogels constituting polymers, (in particular, polyampholyte\u2010based polymers) which have inherent efficiency to stabilize proteins and protect them from denaturation. Our findings can open up new avenues in protein biopharmaceutics for the design of materials that can store therapeutic proteins long\u2010term under severe stress and safely deliver them.<\/jats:p>","DOI":"10.1002\/adhm.202203253","type":"journal-article","created":{"date-parts":[[2023,2,23]],"date-time":"2023-02-23T02:13:09Z","timestamp":1677118389000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Polyampholyte\u2010Based Polymer Hydrogels for the Long\u2010Term Storage, Protection and Delivery of Therapeutic Proteins"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6610-9661","authenticated-orcid":false,"given":"Robin","family":"Rajan","sequence":"first","affiliation":[{"name":"School of Materials Science Japan Advanced Institute of Science and Technology  1\u20101 Asahidai, Nomi 923\u20131292 Ishikawa 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