{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T12:06:08Z","timestamp":1787313968337,"version":"build-2736575974"},"reference-count":79,"publisher":"Wiley","issue":"21","license":[{"start":{"date-parts":[[2023,4,29]],"date-time":"2023-04-29T00:00:00Z","timestamp":1682726400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["RGPIN\u20102018\u201006209"],"award-info":[{"award-number":["RGPIN\u20102018\u201006209"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["RGPN\u20102016\u201006532"],"award-info":[{"award-number":["RGPN\u20102016\u201006532"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Proteinaceous nanoparticles constitute efficient antigen delivery systems in vaccine formulations due to their size and repetitive nature that mimic most invading pathogens and promote immune activation. Nonetheless, the coadministration of an adjuvant with subunit nanovaccines is usually required to induce a robust, long\u2010lasting, and protective immune response. Herein, the protein Curli\u2010specific gene A (CsgA), which is known to self\u2010assemble into nanofilaments contributing to bacterial biofilm, is exploited to engineer an intrinsically immunostimulatory antigen delivery platform. Three repeats of the M2e antigenic sequence from the influenza A virus matrix 2 protein are merged to the N\u2010terminal domain of engineered CsgA proteins. These chimeric 3M2e\u2010CsgA spontaneously self\u2010assemble into antigen\u2010displaying cross\u2010\n                    <jats:italic>\u03b2<\/jats:italic>\n                    \u2010sheet nanofilaments that activate the heterodimeric toll\u2010like receptors 2 and 1. The resulting nanofilaments are avidly internalized by antigen\u2010presenting cells and stimulate the maturation of dendritic cells. Without the need of any additional adjuvants, both assemblies show robust humoral and cellular immune responses, which translate into complete protection against a lethal experimental infection with the H1N1 influenza virus. Notably, these CsgA\u2010based nanovaccines induce neither overt systemic inflammation, nor reactogenicity, upon mice inoculation. These results highlight the potential of engineered CsgA nanostructures as self\u2010adjuvanted, safe, and versatile antigen delivery systems to fight infectious diseases.\n                  <\/jats:p>","DOI":"10.1002\/adhm.202300224","type":"journal-article","created":{"date-parts":[[2023,4,8]],"date-time":"2023-04-08T22:22:20Z","timestamp":1680992540000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Engineered Curli Nanofilaments as a Self\u2010Adjuvanted Antigen Delivery Platform"],"prefix":"10.1002","volume":"12","author":[{"given":"F\u00e9lix","family":"Lamontagne","sequence":"first","affiliation":[{"name":"Department of Chemistry Universit\u00e9 du Qu\u00e9bec \u00e0 Montr\u00e9al  C.P.8888, Succursale Centre\u2010Ville Montreal H3C 3P8 Canada"},{"name":"Quebec Network for Research on Protein Function Engineering and Applications (PROTEO)  Quebec H3C 3P8 Canada"},{"name":"The Swine and Poultry Infectious 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