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The long period of inflammation and dysfunctional angiogenesis are the main reasons for wound\u2010healing difficulty in diabetes. In this study, a multifunctional double\u2010layer microneedle (DMN) is constructed to control infection and promote angiogenesis, meeting the multiple demands of the healing process of a diabetic wound. The double\u2010layer microneedle is consisted in a hyaluronic acid substrate and a mixture of carboxymethyl chitosan and gelatin as the tip. The antibacterial drug tetracycline hydrochloride (TH) is loaded into the substrate of the microneedle to achieve rapid sterilization and promote resistance to external bacterial infections. The microneedle tip loaded with recombinant human epidermal growth factor (rh\u2010EGF) is inserted into the skin, in response to gelatinase produced by resident microbe and disassociate to achieve the enzymatic response release. The double\u2010layer drug\u2010loaded microneedles (DMN@TH\/rh\u2010EGF) have antibacterial and antioxidant effects, and promote cell migration and angiogenesis in vitro. In an in vivo diabetic wound model, using rats, the DMN@TH\/rh\u2010EGF patch is able to inhibit inflammation, promote angiogenesis, collagen deposition, and tissue regeneration during the wound healing process, promoting its healing.<\/jats:p>","DOI":"10.1002\/adhm.202300297","type":"journal-article","created":{"date-parts":[[2023,4,28]],"date-time":"2023-04-28T05:55:20Z","timestamp":1682661320000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":94,"title":["Multifunctional Double\u2010Layer and Dual Drug\u2010Loaded Microneedle Patch Promotes Diabetic Wound Healing"],"prefix":"10.1002","volume":"12","author":[{"given":"Wei","family":"Liu","sequence":"first","affiliation":[{"name":"Institute of Biomedical Engineering College of Medicine Southwest Jiaotong University  Chengdu 610031 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingxing","family":"Zhai","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Engineering College of Medicine Southwest Jiaotong University  Chengdu 610031 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue","family":"Zhao","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Engineering College of Medicine Southwest Jiaotong University  Chengdu 610031 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongjie","family":"Cai","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Engineering College of Medicine Southwest Jiaotong University  Chengdu 610031 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinmei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Engineering College of Medicine Southwest Jiaotong University  Chengdu 610031 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Xu","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Engineering College of Medicine Southwest Jiaotong University  Chengdu 610031 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Weng","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Engineering College of Medicine Southwest Jiaotong University  Chengdu 610031 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianshu","family":"Li","sequence":"additional","affiliation":[{"name":"College of Polymer Science and Engineering Sichuan University  Chengdu 610065 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1835-6825","authenticated-orcid":false,"given":"Xingyu","family":"Chen","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Engineering College of Medicine Southwest Jiaotong University  Chengdu 610031 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,5,16]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2017.00904"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.7b01850"},{"key":"e_1_2_8_3_1","volume":"10","author":"Mart\u00ed\u2010Carvajal A. 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