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This study aims to develop a novel DNA aptamer for the diagnosis and treatment of BC using a tissue\u2010based systematic evolution of ligands by an exponential enrichment (SELEX) process. By using SELEX, this work successfully generates\u00a0a new aptamer named TB\u20105, which demonstrates a remarkable and specific affinity for nucleolin (NCL) in BC tissues and displays marked biocompatibility both in vitro and in vivo. Additionally, this work shows that\u00a0NCL is a reliable tissue\u2010specific biomarker in BC. Moreover, according to circular dichroism spectroscopy, TB\u20105 forms a non\u2010G\u2010quadruplex structure, distinguishing it from the current NCL\u2013targeting\u00a0aptamer AS1411, and exhibits a distinct binding region on NCL compared to AS1411. Notably, this study further reveals that TB\u20105 activates NCL function by promoting autophagy and suppressing the migration and invasion of BC cells, which occurs by disrupting mRNA transcription processes. These findings highlight the critical role of NCL in the pathological examination of BC and warrant more comprehensive investigations on anti\u2010NCL aptamers in BC imaging and treatment.<\/jats:p>","DOI":"10.1002\/adhm.202300791","type":"journal-article","created":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T13:32:25Z","timestamp":1685626345000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["A Non\u2010G\u2010Quadruplex DNA Aptamer Targeting NCL for Diagnosis and Therapy in Bladder Cancer"],"prefix":"10.1002","volume":"12","author":[{"given":"Yunyi","family":"Liu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Chemo\/Biosensing and Chemometrics College of Biology Molecular Science and Biomedicine Laboratory and Aptamer Engineering Center of Hunan Province Hunan University  Changsha Hunan 410082 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bei","family":"Hu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Chemo\/Biosensing and Chemometrics College of Biology Molecular Science and Biomedicine Laboratory and Aptamer Engineering Center of Hunan Province Hunan University  Changsha Hunan 410082 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaming","family":"Pei","sequence":"additional","affiliation":[{"name":"Department of Urology Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine. 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