{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,15]],"date-time":"2026-08-15T19:57:48Z","timestamp":1786823868738,"version":"build-2736575974"},"reference-count":42,"publisher":"Wiley","issue":"25","license":[{"start":{"date-parts":[[2023,5,1]],"date-time":"2023-05-01T00:00:00Z","timestamp":1682899200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["DFG WE 5745\/2\u20101"],"award-info":[{"award-number":["DFG WE 5745\/2\u20101"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Vitamin B12 (cobalamin) is essential for human health and its deficiency results in anemia and neurological damage. Vitamin B12 exists in different forms with various bioactivity but most sensors are unable to discriminate between them. Here, a whole\u2010cell agglutination assay that is specific for adenosylcobalamin (AboB12), which is one of two bioactive forms, is reported. This biosensor consists of <jats:italic>Escherichia coli<\/jats:italic> that express the AdoB12 specific binding domain of CarH at their surface. In the presence of AdoB12, CarH forms tetramers, which leads to specific bacterial cell\u2013cell adhesions and agglutination. These CarH tetramers disassemble upon green light illumination such that reversion of the bacterial aggregation can serve as internal quality control. The agglutination assay has a detection limit of 500 n\u043c AdoB12, works in protein\u2010poor biofluids such as urine, and has high specificity to AdoB12 over other forms of vitamin B12 as also demonstrated with commercially available supplements. This work is a proof of concept for a cheap and easy\u2010to\u2010readout AdoB12 sensor that can be implemented at the point\u2010of\u2010care to monitor high\u2010dose vitamin B12 supplementation.<\/jats:p>","DOI":"10.1002\/adhm.202300835","type":"journal-article","created":{"date-parts":[[2023,4,18]],"date-time":"2023-04-18T02:20:22Z","timestamp":1681784422000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["An Adenosylcobalamin Specific Whole\u2010Cell Biosensor"],"prefix":"10.1002","volume":"12","author":[{"given":"Juan Jos\u00e9","family":"Quispe Haro","sequence":"first","affiliation":[{"name":"Institute of Physiological Chemistry and Pathobiochemistry University of M\u00fcnster  Waldeyerstrasse 15 48149 M\u00fcnster Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9072-0858","authenticated-orcid":false,"given":"Seraphine V.","family":"Wegner","sequence":"additional","affiliation":[{"name":"Institute of Physiological Chemistry and Pathobiochemistry University of M\u00fcnster  Waldeyerstrasse 15 48149 M\u00fcnster Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,5]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2022.972468"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu14081672"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/15563650.2019.1606432"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V7.2.243.243"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.metabol.2019.153992"},{"key":"e_1_2_8_6_1","first-page":"269","volume":"11","author":"Hanna S.","year":"2009","journal-title":"J. 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