{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T15:32:40Z","timestamp":1785339160258,"version":"3.55.0"},"reference-count":42,"publisher":"Wiley","issue":"26","license":[{"start":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T00:00:00Z","timestamp":1685577600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T00:00:00Z","timestamp":1685577600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Thiol\u2010norbornene photoclickable poly (ethylene glycol) (PEG)\u2010based (PEG\u2010NB) hydrogels are attractive biomaterials for cell encapsulation, drug delivery, and regenerative medicine applications. Although many crosslinking strategies and chemistries have been developed for PEG\u2010NB bulk hydrogels, fabrication approaches of PEG\u2010NB microgels have not been extensively explored. Here, a fabrication strategy for 4\u2010arm amide\u2010linked PEG\u2010NB (PEG\u20104aNB) microgels using flow\u2010focusing microfluidics for human mesenchymal stem\/stromal cell (hMSCs) encapsulation is presented. PEG\u20104aNB photochemistry allows high\u2010throughput, ultrafast generation, and cost\u2010effective synthesis of monodispersed microgels (diameter 340 \u00b1 18, 380 \u00b1 24, and 420 \u00b1 15\u00a0\u00b5m, for 6, 8, and 10 wt% of PEG\u20104aNB, respectively) using an in situ crosslinking methodology in a microfluidic device. PEG\u20104aNB microgels show in vitro degradability due to the incorporation of a protease\u2010degradable peptide during photocrosslinking and encapsulated cells show excellent viability and metabolic activity for at least 13 days of culture. Furthermore, the secretory profile (i.e., MMP\u201013, ICAM\u20101, PD\u2010L1, CXCL9, CCL3\/MIP\u20101, IL\u20106, IL\u201012, IL\u201017E, TNF\u2010\n                    <jats:italic>\u03b1<\/jats:italic>\n                    , CCL2\/MCP\u20101) of encapsulated hMSCs shows increased expression in response to IFN\u2010\n                    <jats:italic>\u03b3<\/jats:italic>\n                    stimulation. Collectively, this work shows a versatile and facile approach for the fabrication of protease\u2010degradable PEG\u20104aNB microgels for cell encapsulation.\n                  <\/jats:p>","DOI":"10.1002\/adhm.202300942","type":"journal-article","created":{"date-parts":[[2023,5,26]],"date-time":"2023-05-26T16:09:34Z","timestamp":1685117374000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Rapid and Facile Light\u2010Based Approach to Fabricate Protease\u2010Degradable Poly(ethylene glycol)\u2212norbornene Microgels for Cell Encapsulation"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4366-6860","authenticated-orcid":false,"given":"Ana","family":"Mora\u2010Boza","sequence":"first","affiliation":[{"name":"Woodruff School of Mechanical Engineering Georgia Institute of Technology  Atlanta GA 30332\u20100363 USA"},{"name":"Petit Institute for Bioengineering and Bioscience Georgia Institute of Technology  Atlanta GA 30332\u20100363 USA"},{"name":"C\u00daRAM University of Galway  Galway H91 W2TY Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saron G.","family":"Ghebrezadik","sequence":"additional","affiliation":[{"name":"Petit Institute for Bioengineering and Bioscience Georgia Institute of Technology  Atlanta GA 30332\u20100363 USA"},{"name":"Agnes Scott College  Decatur GA 30030 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johannes E.","family":"Leisen","sequence":"additional","affiliation":[{"name":"Petit Institute for Bioengineering and Bioscience Georgia Institute of Technology  Atlanta GA 30332\u20100363 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6602-2518","authenticated-orcid":false,"given":"Andr\u00e9s J.","family":"Garc\u00eda","sequence":"additional","affiliation":[{"name":"Woodruff School of Mechanical Engineering Georgia Institute of Technology  Atlanta GA 30332\u20100363 USA"},{"name":"Petit Institute for Bioengineering and Bioscience Georgia Institute of Technology  Atlanta GA 30332\u20100363 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