{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T01:13:23Z","timestamp":1790298803562,"version":"4.1.0"},"reference-count":34,"publisher":"Wiley","issue":"28","license":[{"start":{"date-parts":[[2023,9,10]],"date-time":"2023-09-10T00:00:00Z","timestamp":1694304000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["52103129"],"award-info":[{"award-number":["52103129"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2023,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Silk fibroin derived from the domesticated silkworm <jats:italic>Bombyx mori<\/jats:italic> is a protein\u2010based biopolymer with low immunogenicity, intrinsic biodegradability, and tunable mechanical properties, showing great potential in biomedical applications. Using chemical modification to alter the primary structure of silk fibroin enables the expanded generation of new silk\u2010based biomaterials. Inspired by the molecular structure of hyaluronic acid, which is enriched in carboxyl groups, an efficient method with scaling\u2010up potential to achieve controlled carboxylation of silk fibroin to prepare silk acid (SA) is reported, and the biological properties of SA are further studied. The SA materials show tunable hydrophilicity and enzymatic degradation properties at different carboxylation degrees (CDs). Subcutaneous implantation in mice for up to 1 month reveals that the SA materials with a high CD present enhanced degradation while causing a mild foreign\u2010body response, including a low inflammatory response and reduced fibrotic encapsulation. Immunofluorescence analysis further indicates that the SA materials show pro\u2010angiogenesis properties and promote M2\u2010type macrophage polarization to facilitate tissue regeneration. This implies great promise for SA materials as a new implantable biomaterial for tissue regeneration.<\/jats:p>","DOI":"10.1002\/adhm.202301439","type":"journal-article","created":{"date-parts":[[2023,8,30]],"date-time":"2023-08-30T17:34:37Z","timestamp":1693416877000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Silk Acid as an Implantable Biomaterial for Tissue Regeneration"],"prefix":"10.1002","volume":"12","author":[{"given":"Ziyang","family":"Sun","sequence":"first","affiliation":[{"name":"School of Engineering Westlake University  Hangzhou Zhejiang 310030 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruochuan","family":"Huang","sequence":"additional","affiliation":[{"name":"School of Engineering Westlake University  Hangzhou Zhejiang 310030 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Lyu","sequence":"additional","affiliation":[{"name":"School of Engineering Westlake University  Hangzhou Zhejiang 310030 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Yu","sequence":"additional","affiliation":[{"name":"School of Engineering Westlake University  Hangzhou Zhejiang 310030 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenzhao","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Engineering Westlake University  Hangzhou Zhejiang 310030 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinghang","family":"Li","sequence":"additional","affiliation":[{"name":"School of Engineering Westlake University  Hangzhou Zhejiang 310030 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingyu","family":"Lu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Precise Synthesis of Functional Molecules of Zhejiang Province Instrumentation and Service Center for Molecular Sciences Westlake University  Hangzhou Zhejiang 310024 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9253-3469","authenticated-orcid":false,"given":"Chengchen","family":"Guo","sequence":"additional","affiliation":[{"name":"School of Engineering Westlake University  Hangzhou Zhejiang 310030 China"},{"name":"Research Center for Industries of the Future Westlake University  Hangzhou Zhejiang 310030 China"},{"name":"Westlake Laboratory of Life Sciences and Biomedicine  Hangzhou Zhejiang 310024 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,9,10]]},"reference":[{"key":"e_1_2_10_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.progpolymsci.2007.05.013"},{"key":"e_1_2_10_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.progpolymsci.2015.02.001"},{"key":"e_1_2_10_1_3","doi-asserted-by":"publisher","DOI":"10.1126\/science.1188936"},{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1039\/C8CS00187A"},{"key":"e_1_2_10_2_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biomac.0c00090"},{"key":"e_1_2_10_2_3","doi-asserted-by":"publisher","DOI":"10.1038\/s41578-019-0150-z"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2011.379"},{"key":"e_1_2_10_3_2","doi-asserted-by":"publisher","DOI":"10.1063\/1.5091442"},{"key":"e_1_2_10_3_3","doi-asserted-by":"publisher","DOI":"10.1038\/s41563-019-0560-8"},{"key":"e_1_2_10_3_4","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2012.09.043"},{"key":"e_1_2_10_3_5","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2015.03.020"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/bm101422j"},{"key":"e_1_2_10_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2014.09.013"},{"key":"e_1_2_10_4_3","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2015.08.039"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.progpolymsci.2013.09.002"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/adem.201700961"},{"key":"e_1_2_10_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/s42765-022-00144-9"},{"key":"e_1_2_10_6_3","author":"Matthew S. 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