{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T02:34:21Z","timestamp":1790217261108,"version":"4.1.0"},"reference-count":47,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,10,4]],"date-time":"2023-10-04T00:00:00Z","timestamp":1696377600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82071121"],"award-info":[{"award-number":["82071121"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Periodontitis, a chronic infection causing periodontal tissue loss, may be effectively addressed with in situ tissue engineering. Small intestinal submucosa (SIS) offers exceptional biocompatibility and biodegradability but lacks sufficient osteoconductive and osteoinductive properties. This study develops and characterizes SIS coated with hydroxyapatite (SIS\u2010HA) and gelatin methacrylate hydroxyapatite (SIS\u2010Gel\u2010HA) using biomineralization and chemical crosslinking. The impact on periodontal tissue regeneration is assessed by evaluating macrophage immune response and osteogenic differentiation potential of periodontal ligament stem cells (PDLSCs) in vitro and rat periodontal defects in vivo. The jejunum segment, with the highest collagen type I content, is optimal for SIS preparation. SIS retains collagen fiber structure and bioactive factors. Calcium content is 2.21% in SIS\u2010HA and 2.45% in SIS\u2010Gel\u2010HA, with no significant differences in hydrophilicity, physicochemical properties, protein composition, or biocompatibility among SIS, SIS\u2010HA, SIS\u2010Gel, and SIS\u2010Gel\u2010HA. SIS is found to upregulate M2 marker expression, both SIS\u2010HA and SIS\u2010Gel\u2010HA enhance the osteogenic differentiation of PDLSCs through the BMP\u20102\/Smad signaling pathway, and SIS\u2010HA demonstrates superior in vitro osteogenic activity. In vivo, SIS\u2010HA and SIS\u2010Gel\u2010HA yield denser, more mature bones with the highest BMP\u20102 and Smad expression. SIS\u2010HA and SIS\u2010Gel\u2010HA demonstrate enhanced immunity\u2013osteogenesis coupling, representing a promising periodontal tissue regeneration approach.<\/jats:p>","DOI":"10.1002\/adhm.202301479","type":"journal-article","created":{"date-parts":[[2023,9,22]],"date-time":"2023-09-22T20:38:55Z","timestamp":1695415135000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Hydroxyapatite\u2010Coated Small Intestinal Submucosa Membranes Enhanced Periodontal Tissue Regeneration through Immunomodulation and Osteogenesis via BMP\u20102\/Smad Signaling Pathway"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7372-6337","authenticated-orcid":false,"given":"Guoping","family":"Cheng","sequence":"first","affiliation":[{"name":"State Key Laboratory of Oral Diseases Sichuan University  Chengdu Sichuan 610041 China"},{"name":"National Center for Stomatology Sichuan University  Chengdu Sichuan 610041 China"},{"name":"National Clinical Research Center for Oral Diseases Sichuan University  Chengdu 610041 China"},{"name":"Department of Periodontics West China School and Hospital of Stomatology Sichuan University  Chengdu 610041 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shujuan","family":"Guo","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases Sichuan University  Chengdu Sichuan 610041 China"},{"name":"National Center for Stomatology Sichuan University  Chengdu Sichuan 610041 China"},{"name":"National Clinical Research Center for Oral Diseases Sichuan University  Chengdu 610041 China"},{"name":"Department of Periodontics West China School and Hospital of Stomatology Sichuan University  Chengdu 610041 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maoxue","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases Sichuan University  Chengdu Sichuan 610041 China"},{"name":"National Center for Stomatology Sichuan University  Chengdu Sichuan 610041 China"},{"name":"National Clinical Research Center for Oral Diseases Sichuan University  Chengdu 610041 China"},{"name":"Department of Periodontics West China School and Hospital of Stomatology Sichuan University  Chengdu 610041 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shimeng","family":"Xiao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases Sichuan University  Chengdu Sichuan 610041 China"},{"name":"National Center for Stomatology Sichuan University  Chengdu Sichuan 610041 China"},{"name":"National Clinical Research Center for Oral Diseases Sichuan University  Chengdu 610041 China"},{"name":"Department of Periodontics West China School and Hospital of Stomatology Sichuan University  Chengdu 610041 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Jiang","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Biomaterials Sichuan University  Chengdu 610065 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0948-4991","authenticated-orcid":false,"given":"Yi","family":"Ding","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases Sichuan University  Chengdu Sichuan 610041 China"},{"name":"National Center for Stomatology Sichuan University  Chengdu Sichuan 610041 China"},{"name":"National Clinical Research Center for Oral Diseases Sichuan University  Chengdu 610041 China"},{"name":"Department of Periodontics West China School and Hospital of Stomatology Sichuan University  Chengdu 610041 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,10,4]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41368-022-00215-y"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.reth.2022.09.008"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-80608-3"},{"key":"e_1_2_9_4_1","first-page":"8","author":"Galli M.","year":"2021","journal-title":"Plast. 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