{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T17:19:30Z","timestamp":1787505570541,"version":"build-2736575974"},"reference-count":43,"publisher":"Wiley","issue":"25","license":[{"start":{"date-parts":[[2023,8,13]],"date-time":"2023-08-13T00:00:00Z","timestamp":1691884800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000781","name":"European Research Council","doi-asserted-by":"publisher","award":["759425"],"award-info":[{"award-number":["759425"]}],"id":[{"id":"10.13039\/501100000781","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Transplantation of microencapsulated pancreatic cells is emerging as a promising therapy to replenish \u03b2\u2010cell mass lost from auto\u2010immune nature of type I diabetes mellitus (T1DM). This strategy intends to use micrometer\u2010sized microgels to provide immunoprotection to transplanted cells to avoid chronic application of immunosuppression. Clinical application of encapsulation has remained elusive due to often limited production throughputs and body's immunological reactions to implanted materials. This article presents a high\u2010throughput fabrication of monodisperse, non\u2010immunogenic, non\u2010degradable, immunoprotective, semi\u2010permeable, enzymatically\u2010crosslinkable polyethylene glycol\u2010tyramine (PEG\u2010TA) microgels for \u03b2\u2010cell microencapsulation. Monodisperse \u03b2\u2010cell laden microgels of \u2248120\u00a0\u00b5m, with a shell thickness of 20\u00a0\u00b5m are produced using an outside\u2010in crosslinking strategy. Microencapsulated \u03b2\u2010cells rapidly self\u2010assemble into islet\u2010sized spheroids. Immunoprotection of the microencapsulated is demonstrated by inability of FITC\u2010IgG antibodies to diffuse into cell\u2010laden microgels and NK\u2010cell\u00a0inability to kill microencapsulated \u03b2\u2010cells. Multiplexed ELISA analysis on live blood immune reactivity confirms limited immunogenicity. Microencapsulated MIN6\u03b21 spheroids remain glucose responsive for 28 days in vitro, and able to restore normoglycemia 5 days post\u2010implantation in diabetic mice without notable amounts of cell death. In short, PEG\u2010TA microgels effectively protect implanted cells from the host's immune system while being viable and functional, validating this strategy for the treatment of T1DM.<\/jats:p>","DOI":"10.1002\/adhm.202301552","type":"journal-article","created":{"date-parts":[[2023,8,7]],"date-time":"2023-08-07T04:55:52Z","timestamp":1691384152000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Microfluidic Generation of Thin\u2010Shelled Polyethylene Glycol\u2010Tyramine Microgels for Non\u2010Invasive Delivery of Immunoprotected \u03b2\u2010Cells"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1459-7592","authenticated-orcid":false,"given":"Nuno","family":"Ara\u00fajo\u2010Gomes","sequence":"first","affiliation":[{"name":"Department of Developmental BioEngineering, TechMed Centre University of Twente  Drienerlolaan 5 Enschede 7522NB The 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