{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T14:56:21Z","timestamp":1780671381386,"version":"3.54.1"},"reference-count":125,"publisher":"Wiley","issue":"30","license":[{"start":{"date-parts":[[2023,8,24]],"date-time":"2023-08-24T00:00:00Z","timestamp":1692835200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81771047"],"award-info":[{"award-number":["81771047"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["11932014"],"award-info":[{"award-number":["11932014"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31971239"],"award-info":[{"award-number":["31971239"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>It is recognized that the changes in the physical properties of extracellular matrix (ECM) result in fine\u2010tuned cell responses including cell morphology, proliferation and differentiation. In this study, a novel patterned equidistant micropillar substrate based on polydimethylsiloxane (PDMS) is designed to mimic the collagen fiber\u2010like network of the cartilage matrix. By changing the component of the curing agent to an oligomeric base, micropillar substrates with the same topology but different stiffnesses are obtained and it is found that chondrocytes seeded onto the soft micropillar substrate maintain their phenotype by gathering type II collagen and aggrecan more effectively than those seeded onto the stiff micropillar substrate. Moreover, chondrocytes sense and respond to micropillar substrates with different stiffnesses by altering the ECM\u2010cytoskeleton\u2010focal adhesion axis. Further, it is found that the soft substrate\u2010preserved chondrocyte phenotype is dependent on the activation of Wnt\/\u03b2\u2010catenin signaling. Finally, it is indicated that the changes in osteoid\u2010like region formation and cartilage phenotype loss in the stiffened sclerotic area of osteoarthritis cartilage to validate the changes triggered by micropillar substrates with different stiffnesses. This study provides the cell behavior changes that are more similar to those of real chondrocytes at tissue level during the transition from a normal state to a state of osteoarthritis.<\/jats:p>","DOI":"10.1002\/adhm.202301685","type":"journal-article","created":{"date-parts":[[2023,8,19]],"date-time":"2023-08-19T05:45:59Z","timestamp":1692423959000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Biomimetic Fibers Based on Equidistant Micropillar Arrays Determines Chondrocyte Fate via Mechanoadaptability"],"prefix":"10.1002","volume":"12","author":[{"given":"Chenchen","family":"Zhou","sequence":"first","affiliation":[{"name":"State Key Laboratory of Oral Diseases West China Hospital of Stomatology Sichuan University  Chengdu 610064 China"},{"name":"National Clinical Research Center for Oral Diseases West China Hospital of Stomatology Sichuan University  Chengdu 610064 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yueyi","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases West China Hospital of Stomatology Sichuan University  Chengdu 610064 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengmeng","family":"Duan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases West China Hospital of Stomatology Sichuan University  Chengdu 610064 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Medical Informatics Chongqing Medical University  Chongqing 400016 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caixia","family":"Pi","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases West China Hospital of Stomatology Sichuan University  Chengdu 610064 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Demao","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases West China Hospital of Stomatology Sichuan University  Chengdu 610064 China"},{"name":"Institute of Biomedical Engineering West China School of Basic Medical Sciences &amp; Forensic Medicine Sichuan University  Chengdu 610064 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoheng","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Engineering West China School of Basic Medical Sciences &amp; Forensic Medicine Sichuan University  Chengdu 610064 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8156-0322","authenticated-orcid":false,"given":"Jing","family":"Xie","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases West China Hospital of Stomatology Sichuan University  Chengdu 610064 China"},{"name":"National Clinical Research Center for Oral Diseases West China Hospital of Stomatology Sichuan University  Chengdu 610064 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,8,24]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41584-021-00724-w"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41413-021-00173-x"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2010.198"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1177\/0363546518756087"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41584-022-00749-9"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1039\/D2NR00474G"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-matsci-062910-100431"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/term.211"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar380"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40634-014-0008-7"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1177\/002215540205001004"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41413-021-00168-8"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsbiomaterials.0c00367"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2022.885224"},{"key":"e_1_2_9_15_1","first-page":"101","volume":"40","author":"Giannoni P.","year":"2003","journal-title":"Biorheology"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2020.118804"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1302\/2046-3758.108.BJR-2021-0086"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI119316"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nnano.2008.410"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2014.11.012"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2019.119724"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20020435"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2014.01.031"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.b.34575"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1039\/c0lc00426j"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1080\/07388551.2022.2034733"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1088\/1758-5090\/acc761"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcmgh.2017.12.010"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1039\/C6LC00001K"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-physiol-021317-121312"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.9b00808"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2022.121564"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41368-019-0052-3"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.nanolett.3c01739"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2015.05.023"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2018.08.018"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsbiomaterials.0c01434"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2012.10.015"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00379.2019"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2018.11.004"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2020.10.005"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioactmat.2022.07.013"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.6b07097"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsbiomaterials.2c01371"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2021.07.002"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10735-010-9257-7"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1177\/19476035211063858"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12964-023-01069-5"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm.2017.108"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1002\/term.2586"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00013.2019"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12551-018-0466-8"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.9b14670"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12551-013-0124-0"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10555-008-9173-4"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.8b08450"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-cellbio-120319-023657"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrurol.2012.116"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mtbio.2023.100682"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.17174"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2016.65"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41413-022-00239-4"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1093\/abbs\/gmab021"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2016.72"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2010.196"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1038\/ijos.2015.39"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2885.2008.00963.x"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2016.219"},{"key":"e_1_2_9_69_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2023.121804"},{"key":"e_1_2_9_70_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2017.53"},{"key":"e_1_2_9_71_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2020.10.010"},{"key":"e_1_2_9_72_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2012.10.003"},{"key":"e_1_2_9_73_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar425"},{"key":"e_1_2_9_74_1","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines10102477"},{"key":"e_1_2_9_75_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21155399"},{"key":"e_1_2_9_76_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2007.08.004"},{"key":"e_1_2_9_77_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00402-009-0981-y"},{"key":"e_1_2_9_78_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0163552"},{"key":"e_1_2_9_79_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2012.10.002"},{"key":"e_1_2_9_80_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.20101"},{"key":"e_1_2_9_81_1","doi-asserted-by":"publisher","DOI":"10.1053\/joca.1998.0164"},{"key":"e_1_2_9_82_1","doi-asserted-by":"publisher","DOI":"10.1093\/rb\/rbac100"},{"key":"e_1_2_9_83_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780280410"},{"key":"e_1_2_9_84_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bioeng.2.1.691"},{"key":"e_1_2_9_85_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2022.e12656"},{"key":"e_1_2_9_86_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12551-021-00835-4"},{"key":"e_1_2_9_87_1","doi-asserted-by":"publisher","DOI":"10.1038\/35099066"},{"key":"e_1_2_9_88_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.119.4.893"},{"key":"e_1_2_9_89_1","doi-asserted-by":"publisher","DOI":"10.2174\/1573397112666161205103009"},{"key":"e_1_2_9_90_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.maturitas.2019.01.005"},{"key":"e_1_2_9_91_1","doi-asserted-by":"publisher","DOI":"10.3389\/fresc.2022.825147"},{"key":"e_1_2_9_92_1","doi-asserted-by":"publisher","DOI":"10.46497\/ArchRheumatol.2021.8242"},{"key":"e_1_2_9_93_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00011.2015"},{"key":"e_1_2_9_94_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13075-016-1119-6"},{"key":"e_1_2_9_95_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar1704"},{"key":"e_1_2_9_96_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00774-022-01388-6"},{"key":"e_1_2_9_97_1","doi-asserted-by":"publisher","DOI":"10.1080\/01478885.2020.1861908"},{"key":"e_1_2_9_98_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.612440"},{"key":"e_1_2_9_99_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1012743108"},{"key":"e_1_2_9_100_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11906-022-01211-7"},{"key":"e_1_2_9_101_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13239-018-0363-0"},{"key":"e_1_2_9_102_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-020-0767-x"},{"key":"e_1_2_9_103_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioactmat.2022.07.031"},{"key":"e_1_2_9_104_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep20395"},{"key":"e_1_2_9_105_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.52779"},{"key":"e_1_2_9_106_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arr.2021.101315"},{"key":"e_1_2_9_107_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.42.26329"},{"key":"e_1_2_9_108_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2009.11.064"},{"key":"e_1_2_9_109_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.9b07147"},{"key":"e_1_2_9_110_1","volume-title":"Metabolic, Degenerative and Inflammatory Diseases of Bones and Joints","author":"Jaffe H. L.","year":"1972"},{"key":"e_1_2_9_111_1","doi-asserted-by":"publisher","DOI":"10.1177\/1602400327"},{"key":"e_1_2_9_112_1","doi-asserted-by":"publisher","DOI":"10.1302\/0301-620X.35B4.627"},{"key":"e_1_2_9_113_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eats.2014.06.006"},{"key":"e_1_2_9_114_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-0920-1"},{"key":"e_1_2_9_115_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.6b00676"},{"key":"e_1_2_9_116_1","doi-asserted-by":"publisher","DOI":"10.1097\/00003086-200008000-00017"},{"key":"e_1_2_9_117_1","doi-asserted-by":"publisher","DOI":"10.1053\/joca.1998.0129"},{"key":"e_1_2_9_118_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2001611118"},{"key":"e_1_2_9_119_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00135.2022"},{"key":"e_1_2_9_120_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2013.11.070"},{"key":"e_1_2_9_121_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2019.01.008"},{"key":"e_1_2_9_122_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2022.08.062"},{"key":"e_1_2_9_123_1","doi-asserted-by":"publisher","DOI":"10.3724\/abbs.2023086"},{"key":"e_1_2_9_124_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2020.02.032"},{"key":"e_1_2_9_125_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41368-022-00165-5"}],"container-title":["Advanced Healthcare Materials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adhm.202301685","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T14:43:27Z","timestamp":1759761807000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/adhm.202301685"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,8,24]]},"references-count":125,"journal-issue":{"issue":"30","published-print":{"date-parts":[[2023,12]]}},"alternative-id":["10.1002\/adhm.202301685"],"URL":"https:\/\/doi.org\/10.1002\/adhm.202301685","archive":["Portico"],"relation":{},"ISSN":["2192-2640","2192-2659"],"issn-type":[{"value":"2192-2640","type":"print"},{"value":"2192-2659","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,8,24]]},"assertion":[{"value":"2023-05-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-08-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2301685"}}