{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T13:49:56Z","timestamp":1788616196666,"version":"build-2803163510"},"reference-count":44,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,11,20]],"date-time":"2023-11-20T00:00:00Z","timestamp":1700438400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100017950","name":"Shanghai Municipal Health Commission","doi-asserted-by":"publisher","award":["20204Y0355"],"award-info":[{"award-number":["20204Y0355"]}],"id":[{"id":"10.13039\/100017950","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Stem cell tissue engineering is a potential treatment for osteoarthritis. However, the number of stem cells that can be delivered, loss of stem cells during injection, and migration ability of stem cells limit applications of traditional stem cell tissue engineering. Herein, kartogenin (KGN)\u2010loaded poly(lactic<jats:italic>\u2010co\u2010<\/jats:italic>glycolic acid) (PLGA) porous microspheres is first engineered via emulsification, and then anchored with chitosan through the amidation reaction to develop a new porous microsphere (PLGA\u2010CS@KGN) as a stem cell expansion vector. Following 3D co\u2010culture of the PLGA\u2010CS@KGN carrier with mesenchymal stem cells (MSCs), the delivery system is injected into the capsule cavity in situ. In vivo and in vitro experiments show that PLGA\u2010CS microspheres have a high cell\u2010carrying capacity up to 1 \u00d7 10<jats:sup>4<\/jats:sup> mm<jats:sup>\u22123<\/jats:sup> and provide effective protection of MSCs to promote their controlled release in the osteoarthritis microenvironment. Simultaneously, KGN loaded inside the microspheres effectively cooperated with PLGA\u2010CS to induce MSCs to differentiate into chondrocytes. Overall, these findings indicate that PLGA\u2010CS@KGN microspheres held high cell\u2010loading ability, adapt to the migration and expansion of cells, and promote MSCs to express markers associated with cartilage repair. Thus, PLGA\u2010CS@KGN can be used as a potential stem cell carrier for enhancing stem cell therapy in osteoarthritis treatment.<\/jats:p>","DOI":"10.1002\/adhm.202302327","type":"journal-article","created":{"date-parts":[[2023,11,10]],"date-time":"2023-11-10T07:16:46Z","timestamp":1699600606000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Stem Cells Expansion Vector via Bioadhesive Porous Microspheres for Accelerating Articular Cartilage Regeneration"],"prefix":"10.1002","volume":"13","author":[{"given":"Lang","family":"Bai","sequence":"first","affiliation":[{"name":"Department of orthopedics Affiliated Suzhou Hospital of Nanjing Medical University  Suzhou Jiangsu 215008 China"},{"name":"Gusu School Nanjing Medical University  458 Shizi Road Suzhou 215006 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qibin","family":"Han","sequence":"additional","affiliation":[{"name":"Department of orthopedics Affiliated Suzhou Hospital of Nanjing Medical University  Suzhou Jiangsu 215008 China"},{"name":"Gusu School Nanjing Medical University  458 Shizi Road Suzhou 215006 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeyu","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases Shanghai Institute of Traumatology and Orthopaedics Ruijin Hospital Shanghai Jiao Tong University School of Medicine  197 Ruijin 2nd Road Shanghai 200025 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of orthopedics Affiliated Suzhou Hospital of Nanjing Medical University  Suzhou Jiangsu 215008 China"},{"name":"Gusu School Nanjing Medical University  458 Shizi Road Suzhou 215006 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingwen","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases Shanghai Institute of Traumatology and Orthopaedics Ruijin Hospital Shanghai Jiao Tong University School of Medicine  197 Ruijin 2nd Road Shanghai 200025 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huitong","family":"Ruan","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases Shanghai Institute of Traumatology and Orthopaedics Ruijin Hospital Shanghai Jiao Tong University School of Medicine  197 Ruijin 2nd Road Shanghai 200025 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junliang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery Hainan Hospital of Chinese People's Liberation Army General Hospital  Sanya 572022 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery Hainan Hospital of Chinese People's Liberation Army General Hospital  Sanya 572022 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6938-9582","authenticated-orcid":false,"given":"Wenguo","family":"Cui","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases Shanghai Institute of Traumatology and Orthopaedics Ruijin Hospital Shanghai Jiao Tong University School of Medicine  197 Ruijin 2nd Road Shanghai 200025 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xing","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of orthopedics Affiliated Suzhou Hospital of Nanjing Medical University  Suzhou Jiangsu 215008 China"},{"name":"Gusu School Nanjing Medical University  458 Shizi Road Suzhou 215006 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuefeng","family":"Hao","sequence":"additional","affiliation":[{"name":"Department of orthopedics Affiliated Suzhou Hospital of Nanjing Medical University  Suzhou Jiangsu 215008 China"},{"name":"Gusu School Nanjing Medical University  458 Shizi Road Suzhou 215006 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,11,20]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(21)00208-7"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.n2321"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1136\/bjsports-2020-103671"},{"key":"e_1_2_8_3_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-021-02478-4"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.276.5309.71"},{"key":"e_1_2_8_5_1","doi-asserted-by":"crossref","DOI":"10.1155\/2021\/6663003","volume":"2021","author":"Cao Z.","year":"2021","journal-title":"Biomed Res. Int."},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2018.02.906"},{"key":"e_1_2_8_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/sctm.18-0183"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-018-1591-7"},{"key":"e_1_2_8_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.arthro.2012.11.017"},{"key":"e_1_2_8_7_3","doi-asserted-by":"publisher","DOI":"10.1186\/s12891-015-0714-z"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202104758"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202202658"},{"key":"e_1_2_8_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/rb\/rbz028"},{"key":"e_1_2_8_9_3","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2010.08.018"},{"key":"e_1_2_8_9_4","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.1c20585"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioactmat.2022.01.033"},{"key":"e_1_2_8_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202201661"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41427-021-00339-3"},{"key":"e_1_2_8_11_2","doi-asserted-by":"publisher","DOI":"10.3390\/jfb14010039"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.201901397"},{"key":"e_1_2_8_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2008.09.006"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2014.08.042"},{"key":"e_1_2_8_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(00)00183-6"},{"key":"e_1_2_8_13_3","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2004.12.001"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1215157"},{"key":"e_1_2_8_14_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biomac.0c00623"},{"key":"e_1_2_8_14_3","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2022.01.018"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.201000544"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202006333"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202004307"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.5b08057"},{"key":"e_1_2_8_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2019.115706"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.37358\/RC.17.6.5649"},{"key":"e_1_2_8_19_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12951-020-00651-0"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmat2999"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10565-020-09532-6"},{"key":"e_1_2_8_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioactmat.2021.05.029"},{"key":"e_1_2_8_21_3","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2014.06.013"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2017.02.010"},{"key":"e_1_2_8_22_2","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.5b06663"},{"key":"e_1_2_8_22_3","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2020.09.107"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.95"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2022.121545"}],"container-title":["Advanced Healthcare Materials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adhm.202302327","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T14:17:09Z","timestamp":1759760229000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/adhm.202302327"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,11,20]]},"references-count":44,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2024,1]]}},"alternative-id":["10.1002\/adhm.202302327"],"URL":"https:\/\/doi.org\/10.1002\/adhm.202302327","archive":["Portico"],"relation":{},"ISSN":["2192-2640","2192-2659"],"issn-type":[{"value":"2192-2640","type":"print"},{"value":"2192-2659","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,11,20]]},"assertion":[{"value":"2023-07-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-11-20","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2302327"}}