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Q\u2010VAX<jats:sup>\u00ae<\/jats:sup>, the approved human Q fever vaccine, is a whole cell vaccine associated with safety concerns. Here a safe particulate subunit vaccine candidate is developed that is ambient\u2010temperature stable and can be cost\u2010effectively manufactured. Endotoxin\u2010free <jats:italic>Escherichia coli<\/jats:italic> is bioengineered to efficiently self\u2010assemble biopolymer particles (BPs) that are densely coated with either strings of 18 T\u2010cell epitopes (COX\u2010BP) or two full\u2010length immunodominant antigens (YbgF\u2010BP\u2010Com1) all derived from <jats:italic>C. burnetii<\/jats:italic>. BP vaccine candidates are ambient\u2010temperature stable. Safety and immunogenicity are confirmed in mice and guinea pig (GP) models. YbgF\u2010BP\u2010Com1 elicits specific and strong humoral immune responses in GPs with IgG titers that are at least 1\u00a0000 times higher than those induced by Q\u2010VAX<jats:sup>\u00ae<\/jats:sup>. BP vaccine candidates are not reactogenic. After challenge with <jats:italic>C. burnetii<\/jats:italic>, YbgF\u2010BP\u2010Com1 vaccine leads to reduced fever responses and pathogen burden in the liver and the induction of proinflammatory cytokines IL\u201012 and IFN\u2010\u03b3 inducible protein (IP\u201010) when compared to negative control groups. These data suggest that YbgF\u2010BP\u2010Com1 induces functional immune responses reducing infection by <jats:italic>C. burnetii<\/jats:italic>. 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