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Herein, the fabrication of anti\u2010infection graphene quantum dots (GQDs) is reported, as a new class of topoisomerase (Topo) targeting nanoantibiotics, by modification of rich N\u2010heterocycles (pyridinic N) at edge sites. The membrane\u2010penetrating, nucleus\u2010localizing, DNA\u2010binding GQDs not only damage the cell walls\/membranes of bacteria or fungi, but also inhibit DNA\u2010binding proteins, such as Topo I, thereby affecting DNA replication, transcription, and recombination. The obtained GQDs exhibit excellent broad\u2010spectrum antimicrobial activity against non\u2010MDR bacteria, MDR bacteria, endospores, and fungi. Beyond combating planktonic microorganisms, GQDs inhibit the formation of biofilms and can kill live bacteria inside biofilms. RNA\u2010seq further demonstrates the upregulation of riboflavin biosynthesis genes, DNA repair related genes, and transport proteins related genes in methicillin\u2010resistant <jats:italic>S. aureus<\/jats:italic> (MRSA) in response to the stress induced by GQDs. In vivo animal experiments indicate that the biocompatible GQDs promote wound healing in MRSA or <jats:italic>C. albicans<\/jats:italic>\u2010infected skin wound models. Thus, GQDs may be a promising antibacterial and antifungal candidate for clinical applications in treating infected wounds and eliminating already\u2010formed biofilms.<\/jats:p>","DOI":"10.1002\/adhm.202302659","type":"journal-article","created":{"date-parts":[[2023,11,27]],"date-time":"2023-11-27T20:27:49Z","timestamp":1701116869000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["N\u2010Heterocycle Modified Graphene Quantum Dots as Topoisomerase Targeted Nanoantibiotics for Combating Microbial Infections"],"prefix":"10.1002","volume":"13","author":[{"given":"Yan","family":"Su","sequence":"first","affiliation":[{"name":"Department of Orthopedic Surgery Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine  Shanghai 200233 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinyan","family":"Hu","sequence":"additional","affiliation":[{"name":"School of 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