{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T15:33:45Z","timestamp":1792424025652,"version":"4.5.0"},"reference-count":59,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2023,11,30]],"date-time":"2023-11-30T00:00:00Z","timestamp":1701302400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Lipid\u2010based nanocarriers have demonstrated high interest in delivering genetic material, exemplified by the success of Onpattro and COVID\u201019 vaccines. While PEGylation imparts stealth properties, it hampers cellular uptake and endosomal escape, and may trigger adverse reactions like accelerated blood clearance (ABC) and hypersensitivity reactions (HSR). This work highlights the great potential of amphiphilic poly(N\u2010methyl\u2010N\u2010vinylacetamide) (PNMVA) derivatives as alternatives to lipid\u2010PEG for siRNA delivery. PNMVA compounds with different degrees of polymerization and hydrophobic segments, are synthesized. Among them, DSPE (1,2\u2010distearoyl\u2010sn\u2010glycero\u20103\u2010phosphoethanolamine)\u2010PNMVA efficiently integrates into lipoplexes and LNP membranes and prevents protein corona formation around these lipid carriers, exhibiting stealth properties comparable to DSPE\u2010PEG. However, unlike DSPE\u2010PEG, DSPE\u2010PNMVA\n                    <jats:sub>24<\/jats:sub>\n                    shows no adverse impact on lipoplexes cell uptake and endosomal escape. In in vivo study with mice, DSPE\u2010PNMVA\n                    <jats:sub>24<\/jats:sub>\n                    lipoplexes demonstrate no liver accumulation, indicating good stealth properties, extended circulation time after a second dose, reduced immunological reaction, and no systemic pro\u2010inflammatory response. Safety of DSPE\u2010PNMVA\n                    <jats:sub>24<\/jats:sub>\n                    is confirmed at the cellular level and in animal models of zebrafish and mice. Overall, DSPE\u2010PNMVA is an advantageous substitute to DSPE\u2010PEG for siRNA delivery, offering comparable stealth and toxicity properties while improving efficacy of the lipid\u2010based carriers by minimizing the dilemma effect and reducing immunological reactions, meaning no ABC or HSR effects.\n                  <\/jats:p>","DOI":"10.1002\/adhm.202302712","type":"journal-article","created":{"date-parts":[[2023,11,23]],"date-time":"2023-11-23T13:33:36Z","timestamp":1700746416000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Poly(\n                    <i>N<\/i>\n                    \u2010methyl\u2010\n                    <i>N<\/i>\n                    \u2010vinylacetamide): A Strong Alternative to PEG for Lipid\u2010Based Nanocarriers Delivering siRNA"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7237-8246","authenticated-orcid":false,"given":"Manon","family":"Berger","sequence":"first","affiliation":[{"name":"Laboratory of Pharmaceutical Technology and Biopharmacy CIRM University of Li\u00e8ge  Avenue Hippocrate 15 Li\u00e8ge 4000 Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7ois","family":"Toussaint","sequence":"additional","affiliation":[{"name":"Center for Education and Research on Macromolecules CERM CESAM Research Unit University of Li\u00e8ge  All\u00e9e du Six Ao\u00fbt, 13 Li\u00e8ge 4000 Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanaa","family":"Ben Djemaa","sequence":"additional","affiliation":[{"name":"Gene Expression and Cancer Laboratory GEC GIGA\u2010Molecular Biology of Diseases University of Li\u00e8ge  Avenue de l'H\u00f4pital 11 Li\u00e8ge 4000 Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erik","family":"Maquoi","sequence":"additional","affiliation":[{"name":"Laboratory of Tumor and Development Biology GIGA\u2010Cancer University of Li\u00e8ge  Avenue Hippocrate, 13 Li\u00e8ge 4000 Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00e9l\u00e8ne","family":"Pendeville","sequence":"additional","affiliation":[{"name":"Platform Zebrafish Facility and Transgenics GIGA University of Li\u00e8ge  Avenue de l'H\u00f4pital 11 Li\u00e8ge 4000 Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brigitte","family":"Evrard","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmaceutical Technology and Biopharmacy CIRM University of Li\u00e8ge  Avenue Hippocrate 15 Li\u00e8ge 4000 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