{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T00:37:52Z","timestamp":1785285472321,"version":"3.55.0"},"reference-count":64,"publisher":"Wiley","issue":"25","license":[{"start":{"date-parts":[[2023,11,27]],"date-time":"2023-11-27T00:00:00Z","timestamp":1701043200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["F30 HL164047"],"award-info":[{"award-number":["F30 HL164047"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000072","name":"National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","award":["R01 DE016523"],"award-info":[{"award-number":["R01 DE016523"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006502","name":"Defense Sciences Office, DARPA","doi-asserted-by":"publisher","award":["W911NF\u201019\u20102\u20100024"],"award-info":[{"award-number":["W911NF\u201019\u20102\u20100024"]}],"id":[{"id":"10.13039\/100006502","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Granular biomaterials have found widespread applications in tissue engineering, in part because of their inherent porosity, tunable properties, injectability, and 3D printability. However, the assembly of granular hydrogels typically relies on spherical microparticles and more complex particle geometries have been limited in scope, often requiring templating of individual microgels by microfluidics or in\u2010mold polymerization. Here, we use dithiolane\u2010functionalized synthetic macromolecules to fabricate photopolymerized microgels via batch emulsion, and then harness the dynamic disulfide crosslinks to rearrange the network. Through unconfined compression between parallel plates in the presence of photoinitiated radicals, we transform the isotropic microgels are transformed into disks. Characterizing this process, we find that the areas of the microgel surface in contact with the compressive plates are flattened while the curvature of the uncompressed microgel boundaries increases. When cultured with C2C12 myoblasts, cells localize to regions of higher curvature on the disk\u2010shaped microgel surfaces. This altered localization affects cell\u2010driven construction of large supraparticle scaffold assemblies, with spherical particles assembling without specific junction structure while disk microgels assemble preferentially on their curved surfaces. These results represent a unique spatiotemporal process for rapid reprocessing of microgels into anisotropic shapes, providing new opportunities to study shape\u2010driven mechanobiological cues during and after granular hydrogel assembly.<\/jats:p>","DOI":"10.1002\/adhm.202302925","type":"journal-article","created":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T07:18:45Z","timestamp":1700551125000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Facile Physicochemical Reprogramming of PEG\u2010Dithiolane Microgels"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6702-079X","authenticated-orcid":false,"given":"Benjamin R.","family":"Nelson","sequence":"first","affiliation":[{"name":"Department of Chemical and Biological Engineering University of Colorado Boulder  Boulder CO 80303 USA"},{"name":"BioFrontiers Institute University of Colorado Boulder  Boulder CO 80303 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2862-3843","authenticated-orcid":false,"given":"Bruce E.","family":"Kirkpatrick","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biological Engineering University of Colorado Boulder  Boulder CO 80303 USA"},{"name":"BioFrontiers Institute University of Colorado Boulder  Boulder CO 80303 USA"},{"name":"Medical Scientist Training Program, School of Medicine University of Colorado Anschutz Medical Campus  Aurora CO 80045 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1424-9037","authenticated-orcid":false,"given":"Nathaniel P.","family":"Skillin","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biological Engineering University of Colorado Boulder  Boulder CO 80303 USA"},{"name":"BioFrontiers Institute University of Colorado Boulder  Boulder CO 80303 USA"},{"name":"Medical Scientist Training Program, School of Medicine University of Colorado Anschutz Medical Campus  Aurora CO 80045 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikolas","family":"Di Caprio","sequence":"additional","affiliation":[{"name":"BioFrontiers Institute University of Colorado Boulder  Boulder CO 80303 USA"},{"name":"Department of Bioengineering University of Pennsylvania  Philadelphia PA 19104 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua S.","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biological Engineering University of Colorado Boulder  Boulder CO 80303 USA"},{"name":"BioFrontiers Institute University of Colorado Boulder  Boulder CO 80303 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lea Pearl","family":"Hibbard","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biological Engineering University of Colorado Boulder  Boulder CO 80303 USA"},{"name":"BioFrontiers Institute University of Colorado Boulder  Boulder CO 80303 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grace K.","family":"Hach","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biological Engineering University of Colorado Boulder  Boulder CO 80303 USA"},{"name":"BioFrontiers Institute University of Colorado Boulder  Boulder CO 80303 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1211-5740","authenticated-orcid":false,"given":"Alex","family":"Khang","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biological Engineering University of Colorado Boulder  Boulder CO 80303 USA"},{"name":"BioFrontiers Institute University of Colorado Boulder  Boulder CO 80303 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy J.","family":"White","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biological Engineering University of Colorado Boulder  Boulder CO 80303 USA"},{"name":"Materials Science and Engineering Program University of Colorado Boulder  Boulder CO 80303 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason A.","family":"Burdick","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biological Engineering University of Colorado Boulder  Boulder CO 80303 USA"},{"name":"BioFrontiers Institute University of Colorado Boulder  Boulder CO 80303 USA"},{"name":"Department of Bioengineering University of Pennsylvania  Philadelphia PA 19104 USA"},{"name":"Materials Science and Engineering Program University of Colorado Boulder  Boulder CO 80303 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher N.","family":"Bowman","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biological Engineering University of Colorado Boulder  Boulder CO 80303 USA"},{"name":"Materials Science and Engineering Program University of Colorado Boulder  Boulder CO 80303 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5725-5691","authenticated-orcid":false,"given":"Kristi S.","family":"Anseth","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biological Engineering University of Colorado Boulder  Boulder CO 80303 USA"},{"name":"BioFrontiers Institute University of Colorado Boulder  Boulder CO 80303 USA"},{"name":"Materials Science and Engineering Program University of Colorado Boulder  Boulder CO 80303 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,11,27]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.201907670"},{"key":"e_1_2_8_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cis.2008.08.008"},{"key":"e_1_2_8_1_3","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201104631"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201308005"},{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/smll.202200951"},{"key":"e_1_2_8_2_3","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202300942"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202301388"},{"key":"e_1_2_8_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/admt.202300209"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsbiomaterials.0c01612"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202206117"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202206110"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0801866105"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202202840"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202200989"},{"key":"e_1_2_8_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202109194"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.202200180"},{"key":"e_1_2_8_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202301597"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/acscentsci.8b00325"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-05300-7"},{"key":"e_1_2_8_11_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aat4634"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201605001"},{"key":"e_1_2_8_12_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.macromol.1c02396"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.2c05113"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsabm.3c00337"},{"key":"e_1_2_8_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201705912"},{"key":"e_1_2_8_14_3","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202202261"},{"key":"e_1_2_8_14_4","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.37540"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.1c00071"},{"key":"e_1_2_8_15_2","doi-asserted-by":"publisher","DOI":"10.1021\/ma200202w"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.7b02759"},{"key":"e_1_2_8_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202204003"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202211209"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201903975"},{"key":"e_1_2_8_18_2","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.1c24095"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.2c08104"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsbiomaterials.2c00370"},{"key":"e_1_2_8_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202001419"},{"key":"e_1_2_8_20_3","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202204301"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/ma2001492"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/adbi.202000280"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202104649"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-36436-w"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.214015"},{"key":"e_1_2_8_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2019.01.006"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioactmat.2021.07.020"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.201301516"},{"key":"e_1_2_8_27_2","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202201921"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abi8157"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2022.121669"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.1c08744"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202105645"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsbiomaterials.6b00218"},{"key":"e_1_2_8_32_2","doi-asserted-by":"publisher","DOI":"10.1039\/D0SM00517G"},{"key":"e_1_2_8_32_3","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2022.07.051"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2208019119"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42254-023-00593-0"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/s43588-023-00551-x"},{"key":"e_1_2_8_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/s42254-023-00617-9"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1088\/1748-605X\/ac7306"},{"key":"e_1_2_8_36_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-38780-3"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202301673"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsbiomaterials.1c01440"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1002392"},{"key":"e_1_2_8_39_2","unstructured":"b)P.Manjunatha \u201cCurvature measure and visualization \u201d GitHub2023 https:\/\/github.com\/preethamam\/CurvatureVisualize\/releases\/tag\/1.0.6."}],"container-title":["Advanced Healthcare Materials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adhm.202302925","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T11:48:26Z","timestamp":1759751306000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/adhm.202302925"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,11,27]]},"references-count":64,"journal-issue":{"issue":"25","published-print":{"date-parts":[[2024,10]]}},"alternative-id":["10.1002\/adhm.202302925"],"URL":"https:\/\/doi.org\/10.1002\/adhm.202302925","archive":["Portico"],"relation":{},"ISSN":["2192-2640","2192-2659"],"issn-type":[{"value":"2192-2640","type":"print"},{"value":"2192-2659","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,11,27]]},"assertion":[{"value":"2023-09-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-11-27","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2302925"}}