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Teeth are anchored to the alveolar bone via the periodontal ligament, which provides stability to the teeth and absorbs mechanical stresses during mastication. Periodontal infection leads to periodontitis, a severe inflammation of the supporting soft tissues that ultimately cause tooth loss. Despite the pressing need of periodontal regeneration for improved oral care, efficient in vitro models of the periodontal tissues are still missing, thus hampering the development of novel, faster, and more effective therapy modalities. Herein, a novel \u201cperiodontal ligament (PDL)\u2010on\u2010chip\u201d model that integrates patient\u2010derived periodontal ligament cells (PDLCs) and endothelial cells is introduced. This microfluidic platform provides optimal conditions for the formation of extensive and perfusable vascular networks. Furthermore, PDLCs elicit blood vessels\u2019 development and maturation while establishing close contacts with the endothelial cells. Potential applications for inflammatory periodontal diseases are also successfully displayed in the \u201cPDL\u2010on\u2010chip\u201d by stimulating inflammation and detecting inflammatory cytokines. This work offers a cornerstone for more complex and specialized microfluidic dental models, which are necessary to unravel complex oral diseases that affect individuals\u2019 general health that go beyond the field of dentistry.<\/jats:p>","DOI":"10.1002\/adhm.202303942","type":"journal-article","created":{"date-parts":[[2024,9,16]],"date-time":"2024-09-16T04:16:35Z","timestamp":1726460195000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["\u201cPeriodontal ligament\u2010on\u2010chip\u201d as a Novel Tool for Studies on the Physiology and Pathology of Periodontal Tissues"],"prefix":"10.1002","volume":"13","author":[{"given":"Sara","family":"Svanberg","sequence":"first","affiliation":[{"name":"ETH Z\u00fcrich  Schanzenstrasse 44 Basel 4056 Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabeth","family":"Hirth","sequence":"additional","affiliation":[{"name":"ETH Z\u00fcrich  Schanzenstrasse 44 Basel 4056 Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thimios A.","family":"Mitsiadis","sequence":"additional","affiliation":[{"name":"Institute of Oral Biology University of Zurich  Plattenstrasse 11 Z\u00fcrich 8032 Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5359-8403","authenticated-orcid":false,"given":"Petra S.","family":"Dittrich","sequence":"additional","affiliation":[{"name":"ETH Z\u00fcrich  Schanzenstrasse 44 Basel 4056 Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,9,16]]},"reference":[{"key":"e_1_2_10_1_1","volume-title":"Ten Cate's Oral Histology Development, Structure, and Function","author":"Nanci A.","year":"2016"},{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjmed.2018.05.042"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2010.01251.x"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2006.05.013"},{"key":"e_1_2_10_5_1","first-page":"72","volume":"11","author":"Nazir M. 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