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This application is based on a well\u2010studied mechanism of binding to the type 1 bacterial pili and, therefore, blocking bacteria adhesion to the uroepithelial cells. To implement D\u2010mannose into carrier systems, the mechanism of action of the sugar in the bladder environment is also relevant and requires investigation. Herein, two different MANNosylation strategies using mesoporous silica nanoparticles (MSNs) are described. The impact of different chemical linkers on bacterial adhesion and bladder cell response is studied via confocal microscopy imaging of the MSN interactions with the respective organisms. Cytotoxicity is assessed and the expression of Toll\u2010like receptor 4 (TLR4) and caveolin\u20101 (CAV\u20101), in the presence or absence of simulated infection with bacterial lipopolysaccharide\u00a0(LPS), is evaluated using the human urinary bladder cancer cell line T24. Further, localisation of the transcription factor NF\u2010\u03baB due to the MANNosylated materials is examined over time. The results show that MANNosylation modifies bacterial adhesion to the nanomaterials and significantly affects TLR4, caveolin\u20101, and NF\u2010\u03baB in bladder cells. These elements are essential components of the inflammatory cascade\/pathogens response during urinary tract infections. These findings demonstrate that MANNosylation is a versatile tool to design hybrid nanocarriers for targeted biomedical applications.<\/jats:p>","DOI":"10.1002\/adhm.202304150","type":"journal-article","created":{"date-parts":[[2024,3,30]],"date-time":"2024-03-30T05:59:52Z","timestamp":1711778392000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["MANNosylation of Mesoporous Silica Nanoparticles Modifies TLR4 Localization and NF\u2010\u03baB Translocation in T24 Bladder Cancer Cells"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8489-693X","authenticated-orcid":false,"given":"Mariam","family":"Hohagen","sequence":"first","affiliation":[{"name":"Department of Functional Materials and Catalysis Faculty of Chemistry University of Vienna  W\u00e4hringer Stra\u00dfe 42 Vienna 1090 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