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This work has previously shown that dexamethasone\u2010pulsed tolerogenic dendritic cells loaded with the arthritis\u2010specific antigen human proteoglycan can suppress arthritis development in a proteoglycan\u2010induced arthritis mouse model. To circumvent ex vivo dendritic cell culture, and enhance antigen\u2010specific effects, drug delivery vehicles, such as liposomes, provide an interesting approach. Here, this work uses anionic 1,2\u2010distearoyl\u2010sn\u2010glycero\u20103\u2010phosphoglycerol liposomes with enhanced loading of human proteoglycan\u2010dexamethasone conjugates by cationic lysine tetramer addition. Antigen\u2010pulsed tolerogenic dendritic cells induced by liposomal dexamethasone in vitro enhanced antigen\u2010specific regulatory T\u00a0cells to a similar extent as dexamethasone\u2010induced tolerogenic dendritic cells. In an inflammatory adoptive transfer model, mice injected with antigen\u2010dexamethasone liposomes have significantly higher antigen\u2010specific type 1 regulatory T\u00a0cells than mice injected with antigen only. The liposomes significantly inhibit the progression of arthritis compared to controls in preventative and therapeutic proteoglycan\u2010induced arthritis mouse models. This coincides with systemic tolerance induction and an increase in\n                    <jats:italic>IL10<\/jats:italic>\n                    expression in the paws of mice. In conclusion,\u00a0a single administration of autoantigen and dexamethasone\u2010loaded liposomes seems to be a promising antigen\u2010specific treatment strategy for arthritis in mice.\n                  <\/jats:p>","DOI":"10.1002\/adhm.202304238","type":"journal-article","created":{"date-parts":[[2024,1,31]],"date-time":"2024-01-31T18:58:05Z","timestamp":1706727485000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Autoantigen\u2010Dexamethasone Conjugate\u2010Loaded Liposomes Halt Arthritis Development in Mice"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3635-9798","authenticated-orcid":false,"given":"Naomi","family":"Benne","sequence":"first","affiliation":[{"name":"Department of Infectious Diseases and Immunology Faculty of Veterinary Medicine Utrecht University  Utrecht 3584 CL The 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