{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T04:49:30Z","timestamp":1771476570390,"version":"3.50.1"},"reference-count":32,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2024,3,10]],"date-time":"2024-03-10T00:00:00Z","timestamp":1710028800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR\u201010\u2010IDEX\u20100002"],"award-info":[{"award-number":["ANR\u201010\u2010IDEX\u20100002"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR\u201020\u2010SFRI\u20100012"],"award-info":[{"award-number":["ANR\u201020\u2010SFRI\u20100012"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010663","name":"H2020 European Research Council","doi-asserted-by":"publisher","award":["95010"],"award-info":[{"award-number":["95010"]}],"id":[{"id":"10.13039\/100010663","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004099","name":"Ligue Contre le Cancer","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004099","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Nanoparticle (NP) surface functionalization with proteins, including monoclonal antibodies (mAbs), mAb fragments, and various peptides, has emerged as a promising strategy to enhance tumor targeting specificity and immune cell interaction. However, these methods often rely on complex chemistry and suffer from batch\u2010dependent outcomes, primarily due to limited control over the protein orientation and quantity on NP surfaces. To address these challenges, a novel approach based on the supramolecular assembly of two peptides is presented to create a heterotetramer displaying V<jats:sub>H<\/jats:sub>Hs on NP surfaces. This approach effectively targets both tumor\u2010associated antigens (TAAs) and immune cell\u2010associated antigens. In vitro experiments showcase its versatility, as various NP types are biofunctionalized, including liposomes, PLGA NPs, and ultrasmall silica\u2010based NPs, and the V<jats:sub>H<\/jats:sub>Hs targeting of known TAAs (HER2 for breast cancer, CD38 for multiple myeloma), and an immune cell antigen (NKG2D for natural killer (NK) cells) is evaluated. In in vivo studies using a HER2+ breast cancer mouse model, the approach demonstrates enhanced tumor uptake, retention, and penetration compared to the behavior of nontargeted analogs, affirming its potential for diverse applications.<\/jats:p>","DOI":"10.1002\/adhm.202304250","type":"journal-article","created":{"date-parts":[[2024,3,6]],"date-time":"2024-03-06T01:55:00Z","timestamp":1709690100000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Supramolecular Heterodimer Peptides Assembly for Nanoparticles Functionalization"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3597-9186","authenticated-orcid":false,"given":"Cl\u00e9lia","family":"Mathieu","sequence":"first","affiliation":[{"name":"Institut de Canc\u00e9rologie Strasbourg Europe  Strasbourg 67000 France"},{"name":"Strasbourg Drug Discovery and Development Institute (IMS)  Strasbourg 67000 France"},{"name":"Equipe labellis\u00e9e ligue contre le cancer  26 Rue d'Ulm Paris 75005 France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shayamita","family":"Ghosh","sequence":"additional","affiliation":[{"name":"Institut de Canc\u00e9rologie Strasbourg Europe  Strasbourg 67000 France"},{"name":"Strasbourg Drug Discovery and Development Institute (IMS)  Strasbourg 67000 France"},{"name":"Equipe labellis\u00e9e ligue contre le cancer  26 Rue d'Ulm Paris 75005 France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julien","family":"Draussin","sequence":"additional","affiliation":[{"name":"Institut de Canc\u00e9rologie Strasbourg Europe  Strasbourg 67000 France"},{"name":"Strasbourg Drug Discovery and Development Institute (IMS)  Strasbourg 67000 France"},{"name":"Equipe labellis\u00e9e ligue contre le cancer  26 Rue d'Ulm Paris 75005 France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adeline","family":"Gasser","sequence":"additional","affiliation":[{"name":"Institut de Canc\u00e9rologie Strasbourg Europe  Strasbourg 67000 France"},{"name":"Strasbourg Drug Discovery and Development Institute (IMS)  Strasbourg 67000 France"},{"name":"Equipe labellis\u00e9e ligue contre le cancer  26 Rue d'Ulm Paris 75005 France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guillaume","family":"Jacquot","sequence":"additional","affiliation":[{"name":"Institut de Canc\u00e9rologie Strasbourg Europe  Strasbourg 67000 France"},{"name":"Strasbourg Drug Discovery and Development Institute (IMS)  Strasbourg 67000 France"},{"name":"Equipe labellis\u00e9e ligue contre le cancer  26 Rue d'Ulm Paris 75005 France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9983-8222","authenticated-orcid":false,"given":"Mainak","family":"Banerjee","sequence":"additional","affiliation":[{"name":"Institut de Canc\u00e9rologie Strasbourg Europe  Strasbourg 67000 France"},{"name":"Strasbourg Drug Discovery and Development Institute (IMS)  Strasbourg 67000 France"},{"name":"Equipe labellis\u00e9e ligue contre le cancer  26 Rue d'Ulm Paris 75005 France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tanushree","family":"Gupta","sequence":"additional","affiliation":[{"name":"Laboratoire de Bioimagerie et Pathologies Universit\u00e9 de Strasbourg  UMR 7021 CNRS Illkirch 67401 France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marc","family":"Schmutz","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Strasbourg CNRS Institut Charles Sadron  UPR 22 Strasbourg 67034 France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C\u00e9line","family":"Mirjolet","sequence":"additional","affiliation":[{"name":"Radiation Oncology Department Preclinical Radiation Therapy and Radiobiology Unit Centre Georges\u2010Fran\u00e7ois Leclerc Unicancer Dijon 21000 France"},{"name":"TIReCS team INSERM UMR 1231 Dijon 21000 France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olivier","family":"Tillement","sequence":"additional","affiliation":[{"name":"Institut Lumi\u00e8re\u2010Mati\u00e8re UMR 5306 Universit\u00e9 Claude Bernard Lyon1\u2010CNRS  Villeurbanne Cedex France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fran\u00e7ois","family":"Lux","sequence":"additional","affiliation":[{"name":"Institut Lumi\u00e8re\u2010Mati\u00e8re UMR 5306 Universit\u00e9 Claude Bernard Lyon1\u2010CNRS  Villeurbanne Cedex France"},{"name":"Institut Universitaire de France (IUF)  Paris 75231 France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2423-830X","authenticated-orcid":false,"given":"Andrey S.","family":"Klymchenko","sequence":"additional","affiliation":[{"name":"Laboratoire de Bioimagerie et Pathologies Universit\u00e9 de Strasbourg  UMR 7021 CNRS Illkirch 67401 France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariel","family":"Donzeau","sequence":"additional","affiliation":[{"name":"Institut de g\u00e9n\u00e9tique et de biologie mol\u00e9culaire et cellulaire  Illkirch 67404 France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xavier","family":"Pivot","sequence":"additional","affiliation":[{"name":"Institut de Canc\u00e9rologie Strasbourg Europe  Strasbourg 67000 France"},{"name":"Strasbourg Drug Discovery and Development Institute (IMS)  Strasbourg 67000 France"},{"name":"Equipe labellis\u00e9e ligue contre le cancer  26 Rue d'Ulm Paris 75005 France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8891-7953","authenticated-orcid":false,"given":"S\u00e9bastien","family":"Harlepp","sequence":"additional","affiliation":[{"name":"Institut de Canc\u00e9rologie Strasbourg Europe  Strasbourg 67000 France"},{"name":"Strasbourg Drug Discovery and Development Institute (IMS)  Strasbourg 67000 France"},{"name":"Equipe labellis\u00e9e ligue contre le cancer  26 Rue d'Ulm Paris 75005 France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9364-1621","authenticated-orcid":false,"given":"Alexandre","family":"Detappe","sequence":"additional","affiliation":[{"name":"Institut de Canc\u00e9rologie Strasbourg Europe  Strasbourg 67000 France"},{"name":"Strasbourg Drug Discovery and Development Institute (IMS)  Strasbourg 67000 France"},{"name":"Equipe labellis\u00e9e ligue contre le cancer  26 Rue d'Ulm Paris 75005 France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,3,10]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41573-020-0090-8"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1039\/c2cs15294k"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nnano.2009.314"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/adtp.202000134"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.drudis.2021.02.017"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aay9249"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202110305"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/adtp.202370024"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1039\/D0NR08191D"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.4161\/mabs.3.5.16089"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1039\/C9NR06512A"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202101565"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2020.01.035"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-01830-8"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1039\/C8BM01208C"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M208528200"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.bioconjchem.9b00276"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.bioconjchem.0c00482"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2010.03.016"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja2084338"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200900942"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1039\/C8TB00195B"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2015.12.053"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/nn800275r"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cocis.2012.09.003"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1039\/c2ra21544f"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19010195"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2013.09.007"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2006.05.016"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-00600-w"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(94)90003-5"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.201805157"}],"container-title":["Advanced Healthcare Materials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adhm.202304250","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T17:13:01Z","timestamp":1759770781000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/adhm.202304250"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,3,10]]},"references-count":32,"journal-issue":{"issue":"15","published-print":{"date-parts":[[2024,6]]}},"alternative-id":["10.1002\/adhm.202304250"],"URL":"https:\/\/doi.org\/10.1002\/adhm.202304250","archive":["Portico"],"relation":{},"ISSN":["2192-2640","2192-2659"],"issn-type":[{"value":"2192-2640","type":"print"},{"value":"2192-2659","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,3,10]]},"assertion":[{"value":"2023-12-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-03-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2304250"}}