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However, the alkyl\u2010hydrazone crosslink lacks stability in biologically relevant environments. Furthermore, when formed with biopolymers such as hyaluronic acid (HA), low molecular weight polymers (&lt;60\u00a0kDa), or low polymer content (&lt;2 wt%) hydrogels are typically employed as entanglements reduce injectability. Here, a high molecular weight (&gt;60\u00a0kDa) HA alkyl\u2010hydrazone crosslinked hydrogel is modified with benzaldehyde\u2010poly(ethylene glycol)<jats:sub>3<\/jats:sub>\u2010azide to incorporate azide functional groups. By reacting azide\u2010modified HA with a multi\u2010arm poly(ethylene glycol) (PEG) functionalized with bicyclononyne, stabilizing triazole bonds are formed through strain\u2010promoted azide\u2010alkyne cycloaddition (SPAAC). Increasing the fraction of triazole bonds within the hydrogel network from 0% to 12% SPAAC substantially increases stability. The slow gelation kinetics of the SPAAC reaction in the 12% SPAAC hydrogel enables transient self\u2010healing properties and a similar extrusion force as the 0% SPAAC hydrogel. Methyl\u2010PEG<jats:sub>4<\/jats:sub>\u2010hydrazide is then introduced to further slowdown network evolution, which temporarily lowers the extrusion force, improves printability, and increases post\u2010extrusion mesenchymal stem cell viability and function in the 12% SPAAC hydrogel. This work demonstrates improved stability and temporal injectability of high molecular weight HA\u2010PEG hydrogels for extrusion\u2010based printing and cell delivery.<\/jats:p>","DOI":"10.1002\/adhm.202400062","type":"journal-article","created":{"date-parts":[[2024,5,28]],"date-time":"2024-05-28T15:28:07Z","timestamp":1716910087000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Engineering a Hydrazone and Triazole Crosslinked Hydrogel for Extrusion\u2010Based Printing and Cell Delivery"],"prefix":"10.1002","volume":"13","author":[{"given":"Matthew W.","family":"Jaeschke","sequence":"first","affiliation":[{"name":"Department of Chemical and Biological Engineering  Boulder CO 80303 USA"},{"name":"BioFrontiers Institute University of Colorado Boulder  Boulder CO 80303 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra N.","family":"Borelli","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biological Engineering  Boulder CO 80303 USA"},{"name":"BioFrontiers Institute University of Colorado Boulder  Boulder CO 80303 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathaniel P.","family":"Skillin","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biological Engineering  Boulder CO 80303 USA"},{"name":"BioFrontiers Institute University of Colorado Boulder  Boulder CO 80303 USA"},{"name":"Medical Scientist Training Program School of Medicine University of Colorado Anschutz Medical Campus  Aurora CO 80045 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy J.","family":"White","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biological Engineering  Boulder CO 80303 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5725-5691","authenticated-orcid":false,"given":"Kristi S.","family":"Anseth","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biological Engineering  Boulder CO 80303 USA"},{"name":"BioFrontiers Institute University of Colorado Boulder  Boulder CO 80303 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,6,3]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2013.03.009"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.22361"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.2c00179"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pmatsci.2020.100738"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201308007"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1088\/1758-5090\/ac1c98"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.201800638"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201303680"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.201605609"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.36323"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202200393"},{"key":"e_1_2_9_12_1","first-page":"265","volume":"01","author":"Narciso N. 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