{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T14:54:44Z","timestamp":1787237684981,"version":"build-2736575974"},"reference-count":52,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,6,19]],"date-time":"2024-06-19T00:00:00Z","timestamp":1718755200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100023581","name":"National Science Foundation Graduate Research Fellowship Program","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100023581","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008590","name":"Dr. Ralph and Marian Falk Medical Research Trust","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008590","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["R01HL145709"],"award-info":[{"award-number":["R01HL145709"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["R01HL150392"],"award-info":[{"award-number":["R01HL150392"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["T32HL007853"],"award-info":[{"award-number":["T32HL007853"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Neutrophils can contribute to inflammatory disease propagation via innate mechanisms intended for inflammation resolution. For example, neutrophil extracellular traps (NETs) are necessary for trapping pathogens but can contribute to clot formation and blood flow restriction, that is, ischemia. Currently, no therapeutics in the clinic directly target NETs despite the known involvement of NETs contributing to mortality and increased disease severity. Vascular\u2010deployed particle\u2010based therapeutics are a novel and robust alternative to traditional small\u2010molecule drugs by enhancing drug delivery to cells of interest. This work designs a high\u2010throughput assay to investigate the immunomodulatory behavior and functionality of salicylic acid\u2010based polymer\u2010based particle therapeutics against NETosis in human neutrophils. Briefly, this work finds that polymeric composition plays a role, and particle size can also influence rates of NETosis. Salicylate\u2010based polymeric (Poly\u2010SA) particles are found to functionally inhibit NETosis depending on the particle size and concentration exposed to neutrophils. This work demonstrates the high throughput method can help fast\u2010track particle\u2010based therapeutic optimization and design, more efficiently preparing this innovative therapeutics for the clinic.<\/jats:p>","DOI":"10.1002\/adhm.202400443","type":"journal-article","created":{"date-parts":[[2024,6,20]],"date-time":"2024-06-20T02:20:41Z","timestamp":1718850041000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Polymerized Salicylic Acid Microparticles Reduce the Progression and Formation of Human Neutrophil Extracellular Traps (NET)s"],"prefix":"10.1002","volume":"14","author":[{"given":"Emma R.","family":"Brannon","sequence":"first","affiliation":[{"name":"Department of Chemical Engineering University of Michigan  2800 Plymouth Road, NCRC B28 Ann Arbor MI 48109 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4919-3312","authenticated-orcid":false,"given":"Logan D.","family":"Piegols","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering University of Michigan  2800 Plymouth Road, NCRC B28 Ann Arbor MI 48109 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2035-5178","authenticated-orcid":false,"given":"Gillian","family":"Cady","sequence":"additional","affiliation":[{"name":"Division of Cardiovascular Medicine Samuel and Jean Frankel Cardiovascular Center University of Michigan  Ann Arbor MI 48109 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9331-1454","authenticated-orcid":false,"given":"Daniel","family":"Kupor","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering University of Michigan  2800 Plymouth Road, NCRC B28 Ann Arbor MI 48109 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueqi","family":"Chu","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering University of Michigan  2800 Plymouth Road, NCRC B28 Ann Arbor MI 48109 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. Valentina","family":"Guevara","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering University of Michigan  2800 Plymouth Road, NCRC B28 Ann Arbor MI 48109 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana R.N.","family":"Lima","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of California Riverside Riverside CA 92521 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5660-5194","authenticated-orcid":false,"given":"Yogendra","family":"Kanthi","sequence":"additional","affiliation":[{"name":"Division of Cardiovascular Medicine Samuel and Jean Frankel Cardiovascular Center University of Michigan  Ann Arbor MI 48109 USA"},{"name":"Section of Vascular Thrombosis &amp; Inflammation Division of Intramural Research National Heart Lung and Blood Institute  Bethesda MD 20892 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7640-6694","authenticated-orcid":false,"given":"David J.","family":"Pinsky","sequence":"additional","affiliation":[{"name":"Division of Cardiovascular Medicine Samuel and Jean Frankel Cardiovascular Center University of Michigan  Ann Arbor MI 48109 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7273-7528","authenticated-orcid":false,"given":"Kathryn E.","family":"Uhrich","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of California Riverside Riverside CA 92521 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8728-6608","authenticated-orcid":false,"given":"Omolola","family":"Eniola\u2010Adefeso","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering University of Michigan  2800 Plymouth Road, NCRC B28 Ann Arbor MI 48109 USA"},{"name":"Department of Biomedical Engineering University of Michigan Ann Arbor MI 48109 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,6,19]]},"reference":[{"key":"e_1_2_10_1_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41578-022-00458-5"},{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1201719"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1134\/S0006297920100065"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3399"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2016.142471"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.5221"},{"key":"e_1_2_10_7_1","first-page":"505","volume":"53","author":"Kobayashi S. D.","year":"2005","journal-title":"Arch. Immunol. Ther. Exp."},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13054-014-0543-8"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12016-020-08804-7"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0028526"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2020.119836"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpha.2022.12.003"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2020.120389"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202001940"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsbiomaterials.7b00831"},{"key":"e_1_2_10_16_1","unstructured":"Protocols and Capabilities from the Nanotechnology Characterization Lab https:\/\/www.cancer.gov\/nano\/research\/ncl\/protocols\u2010capabilities(Accessed: August 2023)."},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-37990-z"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202101534"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-5173(82)90113-2"},{"key":"e_1_2_10_20_1","volume-title":"Image Processing Toolbox version: 11","author":"T. M. Inc","year":"2023"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2019.03.036"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.112.202879"},{"key":"e_1_2_10_23_1","volume-title":"Salicylic Acid (Aspirin)","author":"A. S. Arif H","year":"2023"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-06901-w"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20112322"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2011.04544.x"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aba1474"},{"key":"e_1_2_10_28_1","doi-asserted-by":"crossref","unstructured":"C.Carmona\u2010Rivera M. J.Kaplan Curr. Protoc. Immunol.2016 115 14.41.1.","DOI":"10.1002\/cpim.16"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0176472"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-03257-z"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1700905"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms241512355"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20061383"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-015-8887-8"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.7.2869"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-2952(01)00640-2"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1745-7254.2007.00579.x"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2018.06.001"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1200419109"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20112322"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-09801-x"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2011.04544.x"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI124804"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2022.1071550"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13019-024-02506-3"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202101536"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.02148"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.comtox.2021.100172"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.106.109389"},{"key":"e_1_2_10_50_1","unstructured":"I. T. MathWorks MATLAB version: 9.13.0 (R2022b) The MathWorks Inc. Natick MA2023."},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1111\/jth.14413"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-05-287623"}],"container-title":["Advanced Healthcare Materials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adhm.202400443","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T19:32:45Z","timestamp":1759779165000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/adhm.202400443"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,6,19]]},"references-count":52,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2025,2]]}},"alternative-id":["10.1002\/adhm.202400443"],"URL":"https:\/\/doi.org\/10.1002\/adhm.202400443","archive":["Portico"],"relation":{},"ISSN":["2192-2640","2192-2659"],"issn-type":[{"value":"2192-2640","type":"print"},{"value":"2192-2659","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,6,19]]},"assertion":[{"value":"2024-02-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-06-19","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2400443"}}