{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,13]],"date-time":"2026-11-13T17:24:39Z","timestamp":1794590679421,"version":"4.9.1"},"reference-count":26,"publisher":"Wiley","issue":"22","license":[{"start":{"date-parts":[[2024,5,29]],"date-time":"2024-05-29T00:00:00Z","timestamp":1716940800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Rheumatoid arthritis (RA) is a chronic autoimmune disorder characterized by joint inflammation and destruction. Current treatments, such as Methotrexate (MTX), though effective, often face limitations such as high plasma\n                    <jats:italic>C<\/jats:italic>\n                    <jats:sub>max<\/jats:sub>\n                    and lack of sustained release. This study explores a synergistic approach to RA therapy using folate\u2010liposomal co\u2010delivery of MTX and RELA siRNA (short interfering RNA), targeting RAW264.7 macrophage repolarization via nuclear factor kappa B (NF\u2010\u03baB) pathway inhibition. Extensive in vitro characterizations demonstrate the stability and biocompatibility of this therapy via folate\u2010liposomes. In the collagen\u2010induced arthritis (CIA) rat model, treatment leads to reduced synovial inflammation and improved mobility. The combined MTX and RELA siRNA approach indirectly inhibits inflammatory cytokines, rheumatoid factor (RF), and C\u2010reactive protein (CRP). Targeted macrophage delivery shows marked therapeutic effects in RAW264.7 murine macrophages, potentially modulating M1 to M2 polarization. This research presents a promising avenue for innovative RA therapies by inhibiting the inflammatory cascade and preventing joint damage.\n                  <\/jats:p>","DOI":"10.1002\/adhm.202400679","type":"journal-article","created":{"date-parts":[[2024,5,25]],"date-time":"2024-05-25T02:13:12Z","timestamp":1716603192000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Targeted Macrophage Re\u2010Programming: Synergistic Therapy With Methotrexate and RELA siRNA Folate\u2010Liposome in RAW264.7 Cells and Arthritic Rats"],"prefix":"10.1002","volume":"13","author":[{"given":"Simran","family":"Nasra","sequence":"first","affiliation":[{"name":"Biological and Life Sciences School of Arts &amp; Sciences Ahmedabad University  Central Campus, Navrangpura Ahmedabad Gujarat 380009 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dhiraj","family":"Bhatia","sequence":"additional","affiliation":[{"name":"Biological Engineering Discipline Indian Institute of Technology IIT Gandhinagar  Palaj Gujarat 382355 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6856-3980","authenticated-orcid":false,"given":"Ashutosh","family":"Kumar","sequence":"additional","affiliation":[{"name":"Biological and Life Sciences School of Arts &amp; Sciences Ahmedabad University  Central Campus, Navrangpura Ahmedabad Gujarat 380009 India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,5,29]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.0c13261"},{"key":"e_1_2_9_2_1","first-page":"807","volume":"9","author":"Zyrianova Y.","year":"2012","journal-title":"Rheumatoid arthritis\u2010etiology, consequences and co\u2010morbidities"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehq030"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.5114\/reum.2016.60216"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/2050312117713647"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10067-020-05001-x"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.27503"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s41231-023-00142-y"},{"key":"e_1_2_9_9_1","volume":"14","author":"Mustafa S. 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