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Here, we report a biofilm\u2010penetrating probiotic hybrid strategy for the treatment of periodontitis. It consists of therapeutic probiotics of E. coli Nissle 1917, which can produce antimicrobial peptides and hydrogen, and is coated with D\u2010amino acids that can penetrate biofilms. After the fusion of D\u2010amino acids with the biofilm, EcN entered the plaque biofilm and produced antimicrobial peptides to kill porphyromonas gingivalis and eliminate periodontitis under the action of hydrogen. The efficacy of EcN@DA\u2010D in biofilm penetration and treatment of periodontitis was demonstrated in a rat model of periodontitis. In addition, the clinical combination to construct a rat periodontitis model by using clinical tissue has a significant therapeutic effect. In conclusion, EcN@DA\u2010D offers a promising topical treatment for periodontitis without developing detectable pathogen resistance and side effects.<\/jats:p>","DOI":"10.1002\/adhm.202401190","type":"journal-article","created":{"date-parts":[[2024,10,24]],"date-time":"2024-10-24T00:24:45Z","timestamp":1729729485000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Time\u2010Responsive Activity of Engineered Bacteria for Local Sterilization and Biofilm Removal in Periodontitis"],"prefix":"10.1002","volume":"14","author":[{"given":"Yang","family":"Bai","sequence":"first","affiliation":[{"name":"Department of Stomatology Tianjin Medical University General Hospital  Tianjin 300052 China"},{"name":"Academy of Medical Engineering and Translational Medicine Tianjin University  Tianjin 300072 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao\u2010Lin","family":"Guo","sequence":"additional","affiliation":[{"name":"School of Traditional Chinese Medicine Beijing University of Chinese Medicine  Beijing 102401 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingting","family":"Hua","sequence":"additional","affiliation":[{"name":"Academy of Medical Engineering and Translational Medicine Tianjin University  Tianjin 300072 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bowen","family":"Li","sequence":"additional","affiliation":[{"name":"Academy of Medical Engineering and Translational Medicine Tianjin University  Tianjin 300072 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoqing","family":"Feng","sequence":"additional","affiliation":[{"name":"Academy of Medical Engineering and Translational Medicine Tianjin University  Tianjin 300072 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zijan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Stomatology Tianjin Medical University General Hospital  Tianjin 300052 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Teng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and Epidemiology  Beijing 100071 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Liu","sequence":"additional","affiliation":[{"name":"Tianjin Anding Hospital  Tianjin 300222 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niansong","family":"Qian","sequence":"additional","affiliation":[{"name":"Department of Respiratory the Eighth Medical Center of Chinese PLA General Hospital  Beijing 100853 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0369-5560","authenticated-orcid":false,"given":"Bin","family":"Zheng","sequence":"additional","affiliation":[{"name":"Academy of Medical Engineering and Translational Medicine Tianjin University  Tianjin 300072 China"},{"name":"School of Biomedical Engineering and Technology Tianjin Medical University  Tianjin 300070 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,10,23]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-020-00488-6"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2017.38"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-018-0089-x"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2023.01.008"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2019.06.002"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-15165-4"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-018-0058-4"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1177\/014107687807100301"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12201"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0757.2002.280103.x"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-021-00985-x"},{"key":"e_1_2_9_12_1","first-page":"163","volume":"26","author":"Stower H.","year":"2020","journal-title":"Nat. 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