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Performing high throughput DGEA is challenging due to the high cost and labor\u2010intensive multi\u2010step sample preparation protocols. In particular, performing drug\u2010induced DGEA on cancer cells derived from patient biopsies is even more challenging due to the scarcity of available cells. A novel, miniaturized, nanoliter\u2010scale method for drug\u2010induced DGEA is introduced, enabling high\u2010throughput and parallel analysis of patient\u2010derived cell drug responses, overcoming the limitations and laborious nature of traditional protocols. The method is based on the Droplet Microarray (DMA), a microscope glass slide with hydrophilic spots on a superhydrophobic background, facilitating droplet formation for cell testing. DMA allows microscopy\u2010based phenotypic analysis, cDNA extraction, and DGEA. The procedure includes cell lysis for mRNA isolation and cDNA conversion followed by droplet pooling for qPCR analysis. In this study, the drug\u2010induced DGEA protocol on the DMA platform is demonstrated using patient\u2010derived chronic lymphocytic leukemia (CLL) cells. This methodology is critical for DGEA with limited cell numbers and promise for applications in functional precision oncology. This method enables molecular profiling of patient\u2010derived samples after drug treatment, crucial for understanding individual tumor responses to anticancer drugs.<\/jats:p>","DOI":"10.1002\/adhm.202401820","type":"journal-article","created":{"date-parts":[[2024,10,24]],"date-time":"2024-10-24T00:30:05Z","timestamp":1729729805000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Drug\u2010Induced Differential Gene Expression Analysis on Nanoliter Droplet Microarrays: Enabling Tool for Functional Precision Oncology"],"prefix":"10.1002","volume":"14","author":[{"given":"Razan","family":"El Khaled EL Faraj","sequence":"first","affiliation":[{"name":"Institute of Biological and Chemical Systems\u2010Functional Molecular Systems Karlsruhe Institute of Technology  Hermann\u2010von\u2010Helmholtz\u2010Platz 1 76344 Eggenstein\u2010Leopoldshafen 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