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Consequently, developing accurate cancer research and drug screening models that replicate the structure and function of a fibrosis\u2010surrounded tumor mass is imperative. Previous studies have shown that self\u2010assembly three\u2010dimensional (3D) co\u2010cultures primarily produce cancer\u2010encapsulated fibrosis or maintain a fibrosis\u2010encapsulated tumor mass for a short period, which is inadequate to replicate the function of fibrosis, particularly as a physical barrier. To address this limitation, a multi\u2010layer spheroid formation method is developed to create a fibrosis\u2010encapsulated tumoroid (FET) structure that maintains structural stability for up to 14 days. FETs exhibited faster tumor growth, higher expression of immunosuppressive cytokines, and equal or greater resistance to anticancer drugs compared\u00a0to their parental tumoroids. Additionally, FETs serve as a versatile model for traditional cancer research, enabling the study of exosomal miRNA and gene functions, as well as for mechanobiology research when combined with alginate hydrogel. Our findings suggest that the FET represents an advanced model that more accurately mimics solid cancer tissue with peritumoral fibrosis. It may show potential superiority over self\u2010assembly\u2010based 3D co\u2010cultures for cancer research and drug screening, and holds promise for personalized drug selection in cancer treatment.<\/jats:p>","DOI":"10.1002\/adhm.202402391","type":"journal-article","created":{"date-parts":[[2024,9,5]],"date-time":"2024-09-05T03:12:24Z","timestamp":1725505944000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Fibrosis\u2010Encapsulated Tumoroid, A Solid Cancer Assembloid Model for Cancer Research and Drug Screening"],"prefix":"10.1002","volume":"13","author":[{"given":"Yeonsue","family":"Jang","sequence":"first","affiliation":[{"name":"Department of Urological Science Institute Yonsei University College of Medicine  Seoul 03722 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suki","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Pathology Yonsei 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