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Despite recent advances in bioinspired adhesives to seal the AF defect, the growing popularity of endoscopic discectomy has put forward high requirements for the tissue bioadhesives with rapid injectability, easy operation, and robust tissue adhesion in underwater environments. Herein, a rapidly in situ forming injectable tetra\u2010PEG bioadhesive (ISG) comprising of FDA\u2010approved tetra\u2010armed poly (ethylene glycol) amine (tetra\u2010PEG\u2010NH<jats:sub>2<\/jats:sub>) and tetra\u2010armed poly (ethylene glycol) succinimidyl glutarate (tetra\u2010PEG\u2010SG) for the sutureless closure of AF defects, is reported. Relying on quick ammonolysis reaction between N\u2010hydroxysuccinimide (NHS)\u2010ester of tetra\u2010PEG\u2010SG polymer and amine groups of tetra\u2010PEG\u2010NH<jats:sub>2<\/jats:sub> polymer and tissue proteins, the uniform networks are formed within seconds with easy injection, efficient waterproofness, instant tissue adhesion, and durable compliance. The goat lumbar discectomy model was used to assess the effect of ISG hydrogels in vivo. The results reveal that the resultant ISG bioadhesive can effectively maintain the disc height, fuse with the host tissue, ameliorate IVD degeneration, and retain the initial biomechanics. Together, this study provides an efficient strategy of in situ injectable glue for the minimally invasive treatment of AF defects.<\/jats:p>","DOI":"10.1002\/adhm.202403163","type":"journal-article","created":{"date-parts":[[2024,11,24]],"date-time":"2024-11-24T08:20:36Z","timestamp":1732436436000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Robustly Injectable Tetra\u2010PEG Hydrogel Sealants for Annulus Fibrosus Repair"],"prefix":"10.1002","volume":"14","author":[{"given":"Guoke","family":"Tang","sequence":"first","affiliation":[{"name":"Department of Orthopaedic Tongren Hospital Shanghai Jiao Tong University School of Medicine  1111 Xianxia Road Shanghai 200336 China"},{"name":"Beijing National Laboratory for Molecular Sciences Institute of Chemistry Chinese Academy of Sciences  Beijing 100190 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yucai","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Tongren Hospital Shanghai Jiao Tong University School of Medicine  1111 Xianxia Road Shanghai 200336 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Tongren Hospital Shanghai Jiao Tong University School of Medicine  1111 Xianxia Road Shanghai 200336 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lan","family":"Lin","sequence":"additional","affiliation":[{"name":"Pathology department Tongren Hospital Shanghai Jiao Tong University School of Medicine  1111 Xianxia Road Shanghai 200336 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jielin","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Tongren Hospital Shanghai Jiao Tong University School of Medicine  1111 Xianxia Road Shanghai 200336 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3926-1999","authenticated-orcid":false,"given":"Xing","family":"Wang","sequence":"additional","affiliation":[{"name":"Beijing National Laboratory for Molecular Sciences Institute of Chemistry Chinese Academy of Sciences  Beijing 100190 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojian","family":"Ye","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Tongren Hospital Shanghai Jiao Tong University School of Medicine  1111 Xianxia Road Shanghai 200336 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,11,24]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)32340-0"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/life14070812"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.202300227R"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(21)00733-9"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1503\/cmaj.230542"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/BRS.0000000000002294"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00264-018-4247-6"},{"key":"e_1_2_8_8_1","doi-asserted-by":"crossref","DOI":"10.1136\/bmj-2021-065846","volume":"376","author":"Gadjradj P. 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