{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T16:13:51Z","timestamp":1789661631669,"version":"4.0.1"},"reference-count":63,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,2,17]],"date-time":"2025-02-17T00:00:00Z","timestamp":1739750400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100022720","name":"Fondazione Regionale per la Ricerca Biomedica","doi-asserted-by":"publisher","award":["ID 1731651"],"award-info":[{"award-number":["ID 1731651"]}],"id":[{"id":"10.13039\/501100022720","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002745","name":"Bayerische Forschungsstiftung","doi-asserted-by":"publisher","award":["AZ 802\u201308"],"award-info":[{"award-number":["AZ 802\u201308"]}],"id":[{"id":"10.13039\/501100002745","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002745","name":"Bayerische Forschungsstiftung","doi-asserted-by":"publisher","award":["AZ 1536\u201021"],"award-info":[{"award-number":["AZ 1536\u201021"]}],"id":[{"id":"10.13039\/501100002745","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Advances in understanding the mechanisms behind genetic diseases like Duchenne muscular dystrophy (DMD) underscore the critical role of the extracellular matrix (ECM) composition in disease progression. Effective in vitro models must replicate the intercellular relationships and physicochemical properties of native ECM to fully capture disease\u2010specific characteristics. Although recent biomaterials support the in vitro biofabrication of pathophysiological environments, they often lack disease\u2010specific ECM features. In this study, DystroGel, a hydrogel derived from the cardiac ECM of a porcine DMD model, replicates the distinct molecular composition of dystrophic cardiac tissue for the first time. The findings indicate that the dystrophic ECM matrix exhibits a unique protein profile, impacting cellular processes critical to DMD pathology. This work demonstrates the importance of using a 3D substrate that recreates intercellular dynamics within a defined pathological environment, enhancing the ability to model genetic disorders and providing a valuable tool for advancing personalized therapeutic strategies.<\/jats:p>","DOI":"10.1002\/adhm.202404251","type":"journal-article","created":{"date-parts":[[2025,2,18]],"date-time":"2025-02-18T01:25:20Z","timestamp":1739841920000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Mimicking the Dystrophic Cardiac Extracellular Environment through DystroGel"],"prefix":"10.1002","volume":"14","author":[{"given":"Maila","family":"Chiriv\u00ec","sequence":"first","affiliation":[{"name":"Department of Molecular Medicine Sapienza University  Viale Regina Elena, 324 Rome 00161 Italy"},{"name":"Neurology Unit Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico  Via Francesco Sforza, 35 Milan 20122 Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabio","family":"Maiullari","sequence":"additional","affiliation":[{"name":"Ph.D. Program in Cellular and Molecular Biology Department of Biology University of Rome \u201cTor Vergata\u201d  Via della Ricerca Scientifica, 1 Rome 00133 Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marika","family":"Milan","sequence":"additional","affiliation":[{"name":"Neurology Unit Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico  Via Francesco Sforza, 35 Milan 20122 Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Grazia","family":"Ceraolo","sequence":"additional","affiliation":[{"name":"Neurology Unit Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico  Via Francesco Sforza, 35 Milan 20122 Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole","family":"Fratini","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine Sapienza University  Viale Regina Elena, 324 Rome 00161 Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandra","family":"Fasciani","sequence":"additional","affiliation":[{"name":"Fondazione Istituto Nazionale di Genetica Molecolare \u201cRomeo ed Enrica Invernizzi\u201d  Via Francesco Sforza, 35 Milan 20122 Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salma","family":"Bousselmi","sequence":"additional","affiliation":[{"name":"Neurology Unit Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico  Via Francesco Sforza, 35 Milan 20122 Italy"},{"name":"Ph.D. Program in Cellular and Molecular Biology Department of Biology University of Rome \u201cTor Vergata\u201d  Via della Ricerca Scientifica, 1 Rome 00133 Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Stirm","sequence":"additional","affiliation":[{"name":"Chair for Molecular Animal Breeding and Biotechnology Gene Center and Department of Veterinary Sciences LMU Munich  81377 Munich Germany"},{"name":"Center for Innovative Medical Models (CiMM) Department of Veterinary Sciences LMU Munich  85764 Oberschlei\u00dfheim Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Scalera","sequence":"additional","affiliation":[{"name":"Institute of Nanotechnology National Research Council c\/o Campus Ecotekne via Monteroni  Lecce 73100 Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Gervaso","sequence":"additional","affiliation":[{"name":"Institute of Nanotechnology National Research Council c\/o Campus Ecotekne via Monteroni  Lecce 73100 Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michela","family":"Villa","sequence":"additional","affiliation":[{"name":"Fondazione Istituto Nazionale di Genetica Molecolare \u201cRomeo ed Enrica Invernizzi\u201d  Via Francesco Sforza, 35 Milan 20122 Italy"},{"name":"Department of Biosciences University of Milan  Via Celoria, 26 Milan 20133 Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raffaello","family":"Vigan\u00f2","sequence":"additional","affiliation":[{"name":"Institute for Biomedical Technologies National Research Council  Via Fratelli Cervi, 93, Segrate Milan 20054 Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Brambilla","sequence":"additional","affiliation":[{"name":"Institute for Biomedical Technologies National Research Council  Via Fratelli Cervi, 93, Segrate Milan 20054 Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierluigi","family":"Mauri","sequence":"additional","affiliation":[{"name":"Institute for Biomedical Technologies National Research Council  Via Fratelli Cervi, 93, Segrate Milan 20054 Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"De Falco","sequence":"additional","affiliation":[{"name":"Institute for Biomedical Technologies National Research Council  Via Fratelli Cervi, 93, Segrate Milan 20054 Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dario Di","family":"Silvestre","sequence":"additional","affiliation":[{"name":"Department of Biosciences University of Milan  Via Celoria, 26 Milan 20133 Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Costantini","sequence":"additional","affiliation":[{"name":"Institute of Physical Chemistry \u2013 Polish Academy of Sciences  Marcina Kasprzaka 44\/52 Warsaw 01\u2013224 Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eckhard","family":"Wolf","sequence":"additional","affiliation":[{"name":"Chair for Molecular Animal Breeding and Biotechnology Gene Center and Department of Veterinary Sciences LMU Munich  81377 Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8218-9833","authenticated-orcid":false,"given":"Claudia","family":"Bearzi","sequence":"additional","affiliation":[{"name":"Institute for Biomedical Technologies National Research Council  Via Fratelli Cervi, 93, Segrate Milan 20054 Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6084-7199","authenticated-orcid":false,"given":"Roberto","family":"Rizzi","sequence":"additional","affiliation":[{"name":"Department of Medical\u2010Surgical Sciences and Biotechnologies Sapienza University of Rome  C.so della Repubblica 79 Latina 04100 Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,2,17]]},"reference":[{"key":"e_1_2_10_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacbts.2021.11.004"},{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm9103186"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/brainsci12091190"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1212\/NXI.0000000000000523"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3904"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.114.302533"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.023820"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/febs.15776"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2018.03.023"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a005058"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1088\/1748-3190\/10\/5\/056001"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2014.00203"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13036-022-00292-x"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddu113"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2016.0027"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1088\/1758-5090\/abdacf"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12891-016-1130-8"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2015.12.011"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2015.01.076"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2015.10.076"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.a.2007.0158"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.119.316155"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells9061527"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/gels7020070"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioactmat.2021.09.004"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1242\/dmm.049285"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aaa7322"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcn.2017.01.006"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms4935"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-022-03980-y"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.06.030"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41566-020-0631-z"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.2147\/CMAR.S188216"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1088\/1758-5090\/ace934"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27113442"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1088\/1758-5090\/ab5d80"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2020.116914"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.119.311148"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2020.03.020"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.201801146"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.118.314438"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.5772\/intechopen.96585"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2013.07.023"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2011.85"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11481-014-9566-9"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.progpolymsci.2015.02.004"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioactmat.2021.09.014"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.201605486"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1116995"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcvm.2022.900428"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1177\/147323000903700531"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1524-475X.2009.00466.x"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiosc.2012.10.004"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsabm.0c01490"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2020.103032"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2013.08.015"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10934-022-01309-y"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22189959"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.5560070320"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.8b00702"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.14214"},{"key":"e_1_2_10_62_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells12050699"},{"key":"e_1_2_10_63_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00546.2020"}],"container-title":["Advanced Healthcare Materials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adhm.202404251","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T19:22:55Z","timestamp":1759778575000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/adhm.202404251"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,17]]},"references-count":63,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2025,4]]}},"alternative-id":["10.1002\/adhm.202404251"],"URL":"https:\/\/doi.org\/10.1002\/adhm.202404251","archive":["Portico"],"relation":{},"ISSN":["2192-2640","2192-2659"],"issn-type":[{"value":"2192-2640","type":"print"},{"value":"2192-2659","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,17]]},"assertion":[{"value":"2024-11-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2404251"}}