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Here, a <jats:italic>Brucella<\/jats:italic> BP26 protein\u2010based nanoparticle displaying the MHC class II\u2010restricted melanoma neoantigen, M30, is reported for use as a therapeutic cancer vaccine. Genetic engineering of 10 tandem repeats of the M30 neoepitope to a BP26 monomer results in the self\u2010assembled, neoantigen\u2010displaying protein nanoparticles (BP26\u2010M30 NPs). Subcutaneous immunization of mice with BP26\u2010M30 NPs\/CpG adjuvant leads to the activation and maturation of antigen\u2010presenting cells in draining local lymph nodes and elicits M30\u2010specific CD4<jats:sup>+<\/jats:sup> T cell immune responses and immunological memory. In a mouse model of aggressive B16\u2010F10 melanoma, immunization with BP26\u2010M30 NPs\/CpG significantly inhibits the growth of established tumors. These findings suggest that the BP26\u2010based self\u2010assembled protein nanoparticle has the potential to be used as a cancer vaccine platform for personalized cancer immunotherapy.<\/jats:p>","DOI":"10.1002\/adhm.202404316","type":"journal-article","created":{"date-parts":[[2024,12,23]],"date-time":"2024-12-23T06:41:56Z","timestamp":1734936116000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Neoantigen\u2010Displaying Protein Nanoparticles as a Therapeutic Cancer Vaccine Against Melanoma"],"prefix":"10.1002","volume":"14","author":[{"given":"Yujin","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Biological Sciences KAIST Institute for the BioCentury Korea Advanced Institute of Science and Technology (KAIST)  291 Daehak\u2010ro Daejeon 34141 Republic of Korea"},{"name":"Center for Precision Bio\u2010Nanomedicine Korea Advanced Institute of Science and Technology (KAIST)  291 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