{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T16:05:41Z","timestamp":1787501141387,"version":"build-2736575974"},"reference-count":38,"publisher":"Wiley","issue":"16","license":[{"start":{"date-parts":[[2025,3,7]],"date-time":"2025-03-07T00:00:00Z","timestamp":1741305600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    In 2024, mpox (formerly \u201cmonkeypox\u201d) re\u2010emerged as a global health threat due to the more severe Clade I strain, underscoring the need for rapid, accessible diagnostics. Traditional polymerase chain reaction (PCR) methods, while effective, demand complex laboratory infrastructure and extended processing times. To address these limitations, this study presents a fast\u2010flow micromixer paper\u2010based analytical device (fmPAD) designed for ultrasensitive, rapid mpox virus (MPXV) detection. The device employs a specially engineered sensing probe with a cytosine\u2010cytosine (C\u2500C) mismatch sequence that hybridizes with the MPXV target, reactivating urease previously inhibited by Ag\n                    <jats:sup>+<\/jats:sup>\n                    ions and generating a color change in a pH\u2010sensitive indicator. Enhanced fluid mixing and flow within the fmPAD allow efficient enzymatic reactivation, achieving a linear detection range from 1 to 1000 p\n                    <jats:sc>m<\/jats:sc>\n                    , with a limit of detection (LOD) down to 65 fM. Real viral particles in urine and saliva are also successfully detected at concentrations as low as 5 copies\u00a0mL\n                    <jats:sup>\u22121<\/jats:sup>\n                    without DNA amplification, using a portable colorimeter\u2014representing the lowest LOD among current MPXV DNA sensors. The fmPAD, combined with portable readout technology, enables remote, real\u2010time analysis, offering a practical tool for monitoring and containing the spread of Clade I mpox.\n                  <\/jats:p>","DOI":"10.1002\/adhm.202404320","type":"journal-article","created":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T21:01:47Z","timestamp":1741726907000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Intended Mismatch DNA Nanocomplexes for Ultrasensitive, Enzymatic Colorimetric Detection of MPOX Virus in Minutes Using a Paper\u2010Based Fast\u2010Flow Micromixing Device"],"prefix":"10.1002","volume":"14","author":[{"given":"Lalitphan","family":"Hongtanee","sequence":"first","affiliation":[{"name":"The Institute of Biotechnology and Genetic Engineering Chulalongkorn University  Bangkok 10330 Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sudkate","family":"Chaiyo","sequence":"additional","affiliation":[{"name":"The Institute of Biotechnology and Genetic Engineering Chulalongkorn University  Bangkok 10330 Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongkai","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Chemistry Hong Kong University of Science and Technology  Hong Kong 999077 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8392-5492","authenticated-orcid":false,"given":"Abdulhadee","family":"Yakoh","sequence":"additional","affiliation":[{"name":"The Institute of Biotechnology and Genetic Engineering Chulalongkorn University  Bangkok 10330 Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3,7]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaut.2022.102855"},{"key":"e_1_2_8_1_2","unstructured":"b) World Health Organization (WHO). WHO Director\u2010General declares the ongoing monkeypox outbreak a Public Health Emergency of International ConcernAvailable at:https:\/\/www.who.int\/europe\/news\/item\/23\u201007\u20102022\u2010who\u2010director\u2010general\u2010declares\u2010the\u2010ongoing\u2010monkeypox\u2010outbreak\u2010a\u2010public\u2010health\u2010event\u2010of\u2010international\u2010concern(accessed: July 2024)"},{"key":"e_1_2_8_2_1","unstructured":"World Health Organization (WHO). WHO Director\u2010General declares mpox outbreak a public health emergency of international concern. Available at:https:\/\/www.who.int\/news\/item\/14\u201008\u20102024\u2010who\u2010director\u2010general\u2010declares\u2010mpox\u2010outbreak\u2010a\u2010public\u2010health\u2010emergency\u2010of\u2010international\u2010concern(accessed: July 2024)"},{"key":"e_1_2_8_3_1","unstructured":"World Health Organization (WHO). Multi\u2010country outbreak of mpox External situation report#33\u2010 31 May 2024. Available at:https:\/\/www.who.int\/publications\/m\/item\/multi\u2010country\u2010outbreak\u2010of\u2010mpox\u2010\u2010external\u2010situation\u2010report\u201033\u2010\u201031\u2010may\u20102024(accessed: July 2024)"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2023.106027"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.2446"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/ila2.36"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/JS9.0000000000000115"},{"key":"e_1_2_8_8_1","unstructured":"U.S. Food and Drug Administration (FDA). Monkeypox (mpox) Emergency Use Authorizations for Medical Devices. 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