{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T03:39:07Z","timestamp":1790912347868,"version":"4.1.0"},"reference-count":37,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,2,9]],"date-time":"2025-02-09T00:00:00Z","timestamp":1739059200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,2,9]],"date-time":"2025-02-09T00:00:00Z","timestamp":1739059200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Fungal infections pose a significant global public health threat, particularly candidemia and biofilm formation. Current antifungal drugs have limitations due to their toxicity and drug resistance. Ion interference therapy, particularly cuproptosis, shows significant potential for disease treatment. Herein, nano copper\u2010chelate Cu(DDC)\n                    <jats:sub>2<\/jats:sub>\n                    @BSA (CDB) is synthesized for antifungal research and the mechanism of cuproptosis\u2010like death is investigated. Initially, CDB demonstrates a strong inhibitory effect on multiple fungi and exhibits strong antifungal activity against two fluconazole\u2010resistant clinical isolates. The decrease in ATPase activity and mitochondrial membrane potential indicates that the antifungal mechanism may involve mitochondrial dysfunction. Subsequently, transcriptome analysis reveals significant alterations in genes related to copper ions transport and regulation, oxidative phosphorylation, and mitochondrial function. Additionally, copper ions overload is observed, along with an increase in heat shock protein 70 levels and a decrease in lipoic acid synthetase protein expression. Given that biofilms hinder drug penetration, quaternary ammonium chitosan microneedles are employed in combination with CDB to penetrate the biofilm barrier and enhance the antifungal effect. Overall, this study provides new insight into the cuproptosis\u2010like death mechanism in fungi and presents a promising strategy for fungal infection treatment through the combination of nano copper\u2013chelate and microneedle delivery system.\n                  <\/jats:p>","DOI":"10.1002\/adhm.202404464","type":"journal-article","created":{"date-parts":[[2025,2,11]],"date-time":"2025-02-11T01:55:09Z","timestamp":1739238909000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Nano Copper\u2010chelate Triggers Cuproptosis\u2010like Death in Fungi and Synergizes with Microneedles for Enhanced Biofilm Removal"],"prefix":"10.1002","volume":"14","author":[{"given":"Xing","family":"Ge","sequence":"first","affiliation":[{"name":"College of Pharmacy China Pharmaceutical University  Nanjing 211198 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuyue","family":"Deng","sequence":"additional","affiliation":[{"name":"College of Pharmacy China Pharmaceutical University  Nanjing 211198 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lilin","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Pharmacy China Pharmaceutical University  Nanjing 211198 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Feng","sequence":"additional","affiliation":[{"name":"College of Pharmacy China Pharmaceutical University  Nanjing 211198 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianye","family":"Fang","sequence":"additional","affiliation":[{"name":"College of Pharmacy China Pharmaceutical University  Nanjing 211198 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Ding","sequence":"additional","affiliation":[{"name":"College of Pharmacy China Pharmaceutical University  Nanjing 211198 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Jiang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Detection and Traceability Technology of Foodborne Pathogenic Microorganisms, State Administration for Market Regulation; Key Laboratory of Detection and Traceability Technology of Foodborne Pathogenic Bacteria for Jiangsu Province Market Regulation Nanjing Institute for Food and Drug Control  Nanjing Jiangsu 211198 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Yang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Detection and Traceability Technology of Foodborne Pathogenic Microorganisms, State Administration for Market Regulation; Key Laboratory of Detection and Traceability Technology of Foodborne Pathogenic Bacteria for Jiangsu Province Market Regulation Nanjing Institute for Food and Drug Control  Nanjing Jiangsu 211198 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinmei","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Detection and Traceability Technology of Foodborne Pathogenic Microorganisms, State Administration for Market Regulation; Key Laboratory of Detection and Traceability Technology of Foodborne Pathogenic Bacteria for Jiangsu Province Market Regulation Nanjing Institute for Food and Drug Control  Nanjing Jiangsu 211198 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianjun","family":"Dai","sequence":"additional","affiliation":[{"name":"College of Pharmacy China Pharmaceutical University  Nanjing 211198 P. R. China"},{"name":"MOE Joint International Research Laboratory of Animal Health and Food Safety Key Laboratory of Animal Bacteriology Ministry of Agriculture College of Veterinary Medicine Nanjing Agricultural University  Nanjing 210095 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Pharmacy China Pharmaceutical University  Nanjing 211198 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5158-3846","authenticated-orcid":false,"given":"Yanmin","family":"Ju","sequence":"additional","affiliation":[{"name":"College of Pharmacy China Pharmaceutical University  Nanjing 211198 P. R. 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