{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T06:43:59Z","timestamp":1788417839226,"version":"build-2803163510"},"reference-count":45,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,2,25]],"date-time":"2025-02-25T00:00:00Z","timestamp":1740441600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,2,25]],"date-time":"2025-02-25T00:00:00Z","timestamp":1740441600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32401148"],"award-info":[{"award-number":["32401148"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["52175474"],"award-info":[{"award-number":["52175474"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004543","name":"China Scholarship Council","doi-asserted-by":"publisher","award":["202006890054"],"award-info":[{"award-number":["202006890054"]}],"id":[{"id":"10.13039\/501100004543","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>3D extrusion bioprinting, a promising and widely adopted technology in the emerging field of biofabrication, has gained considerable attention for its ability to fabricate hierarchically structured, native\u2010mimicking tissue substitutes with precisely defined cell distributions. Despite notable advancements, the limited availability of suitably bioactive bioinks remains a major challenge, hindering the construction of volumetric tissue substitutes effectively mimicking biological functionality. Therefore, this work proposes a protein\u2010rich, low\u2010cost, bioactive bioink: abundantly available eggwhite powder (EWP) is leveraged to functionalize an alginate\u2010methylcellulose (AlgMC) hydrogel matrix and enhance cellular response. The developed EWP\u2010supplemented bioinks not only maintain favorable printability and high shape fidelity but also exhibit remarkable bioactivity. Notably, incorporating EWP into AlgMC\u2010based bioinks enhances shear\u2010thinning features, thereby improving the viability of encapsulated cells within the bioprinted constructs. The versatility and biofunctionality of EWP in bioprinted constructs are demonstrated using three distinct cell types, encompassing sources such as a stem cell line, human soft skin, and stiff bone tissues. Furthermore, the promising and wide applicability of the EWP\u2010supplemented bioink for biofabrication is demonstrated exemplarily in core\u2010shell and multi\u2010channel bioprinting strategies as a proof\u2010of\u2010concept for functional tissue construction. These findings underscore the significant and versatile potential of this novel bioink in biofabrication and biomedical applications.<\/jats:p>","DOI":"10.1002\/adhm.202404470","type":"journal-article","created":{"date-parts":[[2025,2,26]],"date-time":"2025-02-26T04:24:11Z","timestamp":1740543851000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Novel Protein\u2010Rich Bioactive Bioink Stimulates Cellular Proliferation and Response in 3D Bioprinted Volumetric Constructs"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5503-2980","authenticated-orcid":false,"given":"Suihong","family":"Liu","sequence":"first","affiliation":[{"name":"Centre for Translational Bone Joint and Soft Tissue Research Faculty of Medicine and University Hospital Carl Gustav Carus Technische Universit\u00e4t Dresden  01307 Dresden Germany"},{"name":"Rapid Manufacturing Engineering Center School of Mechatronic Engineering and Automation Shanghai University  Shanghai 200444 China"},{"name":"Shanghai Key Laboratory of Intelligent Manufacturing and Robotics School of Mechatronic Engineering and Automation Shanghai University  Shanghai 200444 China"},{"name":"Engineering Science and Mechanics Department Penn State University  University Park PA 16802 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3151-3637","authenticated-orcid":false,"given":"David","family":"Kilian","sequence":"additional","affiliation":[{"name":"Centre for Translational Bone Joint and Soft Tissue Research Faculty of Medicine and University Hospital Carl Gustav Carus Technische Universit\u00e4t Dresden  01307 Dresden Germany"},{"name":"Department of Materials Science &amp; Engineering Stanford University  Stanford CA 94305 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1903-1929","authenticated-orcid":false,"given":"Anne","family":"Bernhardt","sequence":"additional","affiliation":[{"name":"Centre for Translational Bone Joint and Soft Tissue Research Faculty of Medicine and University Hospital Carl Gustav Carus Technische Universit\u00e4t Dresden  01307 Dresden Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katharina","family":"Wirsig","sequence":"additional","affiliation":[{"name":"Centre for Translational Bone Joint and Soft Tissue Research Faculty of Medicine and University Hospital Carl Gustav Carus Technische Universit\u00e4t Dresden  01307 Dresden Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Max","family":"von Witzleben","sequence":"additional","affiliation":[{"name":"Centre for Translational Bone Joint and Soft Tissue Research Faculty of Medicine and University Hospital Carl Gustav Carus Technische Universit\u00e4t Dresden  01307 Dresden Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Duin","sequence":"additional","affiliation":[{"name":"Centre for Translational Bone Joint and Soft Tissue Research Faculty of Medicine and University Hospital Carl Gustav Carus Technische Universit\u00e4t Dresden  01307 Dresden Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7704-6435","authenticated-orcid":false,"given":"Anja","family":"Lode","sequence":"additional","affiliation":[{"name":"Centre for Translational Bone Joint and Soft Tissue Research Faculty of Medicine and University Hospital Carl Gustav Carus Technische Universit\u00e4t Dresden  01307 Dresden Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingxi","family":"Hu","sequence":"additional","affiliation":[{"name":"Rapid Manufacturing Engineering Center School of Mechatronic Engineering and Automation Shanghai University  Shanghai 200444 China"},{"name":"Shanghai Key Laboratory of Intelligent Manufacturing and Robotics School of Mechatronic Engineering and Automation Shanghai University  Shanghai 200444 China"},{"name":"National Demonstration Center for Experimental Engineering Training Education Shanghai University  Shanghai 200444 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9075-5121","authenticated-orcid":false,"given":"Michael","family":"Gelinsky","sequence":"additional","affiliation":[{"name":"Centre for Translational Bone Joint and Soft Tissue Research Faculty of Medicine and University Hospital Carl Gustav Carus Technische Universit\u00e4t Dresden  01307 Dresden Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,2,25]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2958"},{"key":"e_1_2_9_2_1","doi-asserted-by":"crossref","DOI":"10.1177\/20417314231187113","volume":"14","author":"Liu S.","year":"2023","journal-title":"J. Tissue Engineer."},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s43586-021-00073-8"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1088\/1758-5090\/aaec52"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201302042"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.0c00084"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioactmat.2023.06.006"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201906423"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10439-019-02337-7"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1039\/C7BM00765E"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajps.2020.10.001"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/term.2058"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmbbm.2020.103642"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1039\/D0BM00027B"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-65050-9"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1369-7021(12)70091-3"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mattod.2020.05.022"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.27954"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1088\/1758-5090\/acb8dc"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s42242-022-00194-3"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1080\/09205063.2020.1864083"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1177\/08853282211001006"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.compositesb.2023.110804"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202315040"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2008.00299.x"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1515\/bmt-2018-0185"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1088\/1758-5090\/aacdc7"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s43246-021-00186-3"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.3390\/gels4030068"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s42242-021-00153-4"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmbbm.2019.103524"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bprint.2022.e00241"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bprint.2020.e00093"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10856-018-6211-9"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1039\/D2BM01499H"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41551-019-0471-7"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.2217\/rme-2018-0022"},{"key":"e_1_2_9_38_1","first-page":"317","volume":"1","author":"Barr\u00e8re F.","year":"2006","journal-title":"Int. J. Nanomed."},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1088\/1758-5090\/ac9019"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1088\/1758-5090\/ac457b"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2020.08.008"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41578-024-00650-9"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2022.08.005"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202304058"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tifs.2024.104670"}],"container-title":["Advanced Healthcare Materials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adhm.202404470","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/adhm.202404470","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adhm.202404470","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T09:09:44Z","timestamp":1770455384000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/adhm.202404470"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,25]]},"references-count":45,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2025,4]]}},"alternative-id":["10.1002\/adhm.202404470"],"URL":"https:\/\/doi.org\/10.1002\/adhm.202404470","archive":["Portico"],"relation":{},"ISSN":["2192-2640","2192-2659"],"issn-type":[{"value":"2192-2640","type":"print"},{"value":"2192-2659","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,25]]},"assertion":[{"value":"2024-11-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-25","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2404470"}}