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Here, a homogeneous nanogrid film composed of Prussian blue\/Au nanogrid (PB\/AuNG) to fabricate salivary biosensors for diagnosing oral diseases is fabricated. The PB\/AuNG nanogrid film allows for the uniform immobilization of oxidase enzymes or antibodies, thereby substantially enhancing the detection sensitivity for trace salivary biomarkers. Then it is designed biosensing chips modified with PB\/AuNG to respectively detect the lactate (potential biomarkers of periodontitis) and interleukin (IL)\u20106 (potential biomarkers of salivary gland tumor). The microchips have achieved the ultra\u2010low limit of 6.5\u00a0\u00b5M to lactate within 5\u00a0s and 0.14\u00a0fg\u00a0mL\n                    <jats:sup>\u22121<\/jats:sup>\n                    to IL\u20106 within 20\u00a0min. After comprehensive performance evaluation and feasibility validation, a handheld saliva analyzer integrated with PB\/AuNG\u2010based microchips for portable, non\u2010hospital\u2010based monitoring is developed. The device is subsequently employed to analyze real saliva samples from periodontitis and salivary gland tumor patients, successfully distinguishing between the two groups based on differences in lactate and IL\u20106 concentrations. This study presents a diagnostic platform for early detection of saliva metabolites, aiming to promote proactive, personalized health management.\n                  <\/jats:p>","DOI":"10.1002\/adhm.202404558","type":"journal-article","created":{"date-parts":[[2025,5,24]],"date-time":"2025-05-24T15:39:15Z","timestamp":1748101155000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["A Handheld Saliva Analyzer Employing Homogeneous Nanogrid Film for Noninvasive on\u2010Site Detection Periodontitis and Salivary Gland Tumor"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2537-7950","authenticated-orcid":false,"given":"Heng","family":"Dong","sequence":"first","affiliation":[{"name":"Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Institute of Stomatology Nanjing University  No.30 Zhongyang Road Nanjing 210008 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Materials\u2010Oriented Chemical Engineering, College of Chemical Engineering Nanjing Tech University  No.30 Puzhu Road Nanjing 211816 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Materials\u2010Oriented Chemical Engineering, College of Chemical Engineering Nanjing Tech University  No.30 Puzhu Road Nanjing 211816 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoye","family":"Li","sequence":"additional","affiliation":[{"name":"Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Institute of Stomatology Nanjing University  No.30 Zhongyang Road Nanjing 210008 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueli","family":"Yuan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Materials\u2010Oriented Chemical Engineering, College of Chemical Engineering Nanjing Tech University  No.30 Puzhu Road Nanjing 211816 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ao","family":"He","sequence":"additional","affiliation":[{"name":"Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Institute of Stomatology Nanjing University  No.30 Zhongyang Road Nanjing 210008 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9086-8502","authenticated-orcid":false,"given":"Qiang","family":"Li","sequence":"additional","affiliation":[{"name":"Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Institute of Stomatology Nanjing University  No.30 Zhongyang Road Nanjing 210008 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8681-3867","authenticated-orcid":false,"given":"Yu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Institute of Stomatology Nanjing University  No.30 Zhongyang Road Nanjing 210008 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8118-7975","authenticated-orcid":false,"given":"Weijun","family":"Xiu","sequence":"additional","affiliation":[{"name":"Institute for Health Innovation &amp; Technology, Biomedical Engineering Department National University of Singapore  21 Lower Kent Ridge Rd Singapore 119077 Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenyu","family":"Chu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Materials\u2010Oriented Chemical Engineering, College of Chemical Engineering Nanjing Tech University  No.30 Puzhu Road Nanjing 211816 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0964-2862","authenticated-orcid":false,"given":"Yongbin","family":"Mou","sequence":"additional","affiliation":[{"name":"Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Institute of Stomatology Nanjing University  No.30 Zhongyang Road Nanjing 210008 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8103-4883","authenticated-orcid":false,"given":"Wanqin","family":"Jin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Materials\u2010Oriented Chemical Engineering, College of Chemical Engineering Nanjing Tech University  No.30 Puzhu Road Nanjing 211816 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,5,24]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40820-024-01602-2"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pan.2024.10.012"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2024.e27437"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1353-8020(13)70025-7"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.2217\/bmm-2016-0232"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11011-022-01114-1"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/962903"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.78872"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12903-024-03902-w"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jobcr.2015.08.006"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12253-019-00588-2"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/2146160"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12337"},{"key":"e_1_2_8_14_1","volume":"8","author":"Rivera C.","year":"2015","journal-title":"Int. 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