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Here, 3D\u2010printed organoid scaffolds for transplantation using clinically relevant human induced pluripotent stem cell\u2010derived regionally specific sNPCs is developed. Scaffolds with microscale channels are printed, and sNPCs are subsequently printed within these channels. The scaffolds direct axonal projections along the channels and guide the cells to simulate in vivo\u2010like conditions, leading to more effective cell maturation and the development of neuronal networks crucial for restoring function after SCI. The scaffolds, with organoids assembled along their lengths, are transplanted into the transected spinal cords of rats. This significantly promotes the functional recovery of the rats. At 12 weeks post\u2010transplantation, the majority of the cells in the scaffolds differentiate into neurons and integrate into the host spinal cord tissue. These results demonstrate their potential to create a relay system along the spinal cord and form synapses in both the rostral and caudal directions relative to the scaffold. It is envisioned that combining sNPCs, organoid assembly, and 3D printing strategies can ultimately lead to a transformative treatment approach for SCI.<\/jats:p>","DOI":"10.1002\/adhm.202404817","type":"journal-article","created":{"date-parts":[[2025,7,24]],"date-time":"2025-07-24T06:45:01Z","timestamp":1753339501000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["3D\u2010Printed Scaffolds Promote Enhanced Spinal Organoid Formation for Use in Spinal Cord Injury"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8808-1607","authenticated-orcid":false,"given":"Guebum","family":"Han","sequence":"first","affiliation":[{"name":"Department of Mechanical Engineering University of Minnesota  Minneapolis MN 55455 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas S.","family":"Lavoie","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery 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