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The success of Masquelet hinges on its ability to promote formation of a \u201cperiosteum\u2010like\u201d FBR\u2010induced membrane at the bone defect site. Inspired by Masquelet's technique, here a novel approach is devised to create periosteum mimetics from decellularized extracellular matrix (dECM), engineered in vivo through FBR, for reconstruction of segmental bone defects. The approach involved 3D printing of polylactic acid (PLA) template with desired pattern\/architecture, followed by subcutaneous implantation of the template to form tissue, and depolymerization and decellularization to generate dECM with interconnected channels. The dECM matrices produces from the same mice (autologous) or from different mice (allogenic) are used as a functional periosteum for repair of structural bone allograft in a murine segmental bone defect model. This study shows that autologous dECM performed better than allogenic dECM, further permitting local delivery of low dose BMP\u20102 to enhance allograft incorporation. The success of this current approach can establish a new line of versatile, patient\u2010specific, and periosteum\u2010like autologous dECM for bone regeneration, offering personalized therapeutics to patients with impaired healing.<\/jats:p>","DOI":"10.1002\/adhm.202404975","type":"journal-article","created":{"date-parts":[[2025,1,22]],"date-time":"2025-01-22T05:51:17Z","timestamp":1737525077000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Masquelet Inspired in Vivo Engineered Extracellular Matrix as Functional Periosteum for Bone Defect Repair and Reconstruction"],"prefix":"10.1002","volume":"14","author":[{"given":"Chen","family":"Jiang","sequence":"first","affiliation":[{"name":"Center for Musculoskeletal Research School of Medicine and Dentistry University of Rochester  Rochester NY 14642 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tianfeng","family":"Miao","sequence":"additional","affiliation":[{"name":"Center for Musculoskeletal Research School of Medicine and Dentistry University of Rochester  Rochester NY 14642 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaojie","family":"Xing","sequence":"additional","affiliation":[{"name":"Center for Musculoskeletal Research School of Medicine and Dentistry University of Rochester  Rochester NY 14642 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kevin J.","family":"Schilling","sequence":"additional","affiliation":[{"name":"Center for Musculoskeletal Research School of Medicine and Dentistry University of Rochester  Rochester NY 14642 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicholas","family":"Lenhard","sequence":"additional","affiliation":[{"name":"Center for Musculoskeletal Research School of Medicine and Dentistry University of Rochester  Rochester NY 14642 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lichen","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Stevens Institute of Technology  Hoboken NJ 07030 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan","family":"McDowell","sequence":"additional","affiliation":[{"name":"Center for Musculoskeletal Research School of Medicine and Dentistry University of Rochester  Rochester NY 14642 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bradley L.","family":"Nilsson","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of Rochester  Rochester NY 14627 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongjun","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Stevens Institute of Technology  Hoboken NJ 07030 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4429-638X","authenticated-orcid":false,"given":"Xinping","family":"Zhang","sequence":"additional","affiliation":[{"name":"Center for Musculoskeletal Research School of Medicine and Dentistry University of Rochester  Rochester NY 14642 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,1,22]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.13075"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/BOT.0000000000001420"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/0363546507308936"},{"key":"e_1_2_9_4_1","unstructured":"a)Medtronic https:\/\/www.medtronic.com\/us\u2010en\/patients\/treatments\u2010therapies\/bone\u2010grafting\u2010spine\u2010orthopaedic\/bone\u2010graft\u2010options\/allograft\u2010tissue.html2021;"},{"key":"e_1_2_9_4_2","unstructured":"b)Grand View Research https:\/\/www.grandviewresearch.com\/industry\u2010analysis\/bone\u2010grafts\u2010substitutes\u2010market2020."},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.3.1274"},{"key":"e_1_2_9_5_2","first-page":"233","volume":"171","author":"Squier C. 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