{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T22:09:05Z","timestamp":1789682945737,"version":"4.0.1"},"reference-count":31,"publisher":"Wiley","issue":"28","license":[{"start":{"date-parts":[[2025,7,29]],"date-time":"2025-07-29T00:00:00Z","timestamp":1753747200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002347","name":"Bundesministerium f\u00fcr Bildung und Forschung","doi-asserted-by":"publisher","award":["13XP5135"],"award-info":[{"award-number":["13XP5135"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["EXC\u20102082\/1\u2013390761711"],"award-info":[{"award-number":["EXC\u20102082\/1\u2013390761711"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Corneal stromal keratocytes (CSK) play a critical role in maintaining corneal transparency and biomechanical integrity. However, challenges in obtaining enough functional CSK limit the progress of corneal tissue engineering and bioprinting. This study presents a novel protocol for the differentiation of bone marrow\u2010derived mesenchymal stromal cells (BM\u2010MSC) into CSK\u2010like cells, optimized for both 2D and 3D culture systems, including 3D bioprinted constructs. A key innovation of this work is the preconditioning of BM\u2010MSC on collagen\u2010coated plates to reduce \u03b1\u2010SMA expression, a marker associated with myofibroblast differentiation and fibrosis. Immunofluorescence and qPCR analyses demonstrated significant upregulation of CSK\u2010specific markers, including Lumican, Keratocan, ALDH1A1, ALDH3A1, and Collagen I, while \u03b1\u2010SMA expression remained undetectable. Notably, 3D cultures provided an enhanced environment for CSK differentiation, mimicking the corneal stroma more closely than 2D cell culture systems. The combination of preconditioning and 3D culture systems offers a promising approach for the biofabrication of functional corneal stroma, paving the way for future therapies aimed at treating corneal opacity and scarring.<\/jats:p>","DOI":"10.1002\/adhm.202405073","type":"journal-article","created":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T06:29:32Z","timestamp":1753856972000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["3D Differentiation of Bone\u2010Marrow Derived Mesenchymal Stromal Cells into the Keratocyte Lineage for Corneal Bioprinting"],"prefix":"10.1002","volume":"14","author":[{"given":"Alexandre","family":"Taoum","sequence":"first","affiliation":[{"name":"Bioprinting &amp; Tissue Engineering Group Center for Molecular Biology of Heidelberg University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronja","family":"Friede","sequence":"additional","affiliation":[{"name":"Bioprinting &amp; Tissue Engineering Group Center for Molecular Biology of Heidelberg University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ole","family":"Thaden","sequence":"additional","affiliation":[{"name":"Bioprinting &amp; Tissue Engineering Group Center for Molecular Biology of Heidelberg University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oleksandr","family":"Rachynskyi","sequence":"additional","affiliation":[{"name":"Bioprinting &amp; Tissue Engineering Group Center for Molecular Biology of Heidelberg University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Frank","sequence":"additional","affiliation":[{"name":"Bioprinting &amp; Tissue Engineering Group Center for Molecular Biology of Heidelberg University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng","family":"Wang","sequence":"additional","affiliation":[{"name":"Bioprinting &amp; Tissue Engineering Group Center for Molecular Biology of Heidelberg University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Friederike","family":"Dehli","sequence":"additional","affiliation":[{"name":"Bioprinting &amp; Tissue Engineering Group Center for Molecular Biology of Heidelberg University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias","family":"Fuest","sequence":"additional","affiliation":[{"name":"Ophthalmology Clinic RWTH Aachen University Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3194-8983","authenticated-orcid":false,"given":"Daniela","family":"Duarte Campos","sequence":"additional","affiliation":[{"name":"Bioprinting &amp; Tissue Engineering Group Center for Molecular Biology of Heidelberg University"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,29]]},"reference":[{"key":"e_1_2_9_1_1","first-page":"459","volume":"9","author":"Spadea L.","year":"2016","journal-title":"Int. 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