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However, conventional ASTs are time\u2010consuming and require pure colonies, delaying the initiation of targeted antimicrobial therapy. Herein, a novel AST based on an aggregation\u2010induced emission luminogen (AIEgen), DATVP, which can directly assess the antimicrobial susceptibility of Gram\u2010negative bacteria in positive blood cultures, is reported. DATVP specifically lights up Gram\u2010negative bacteria with damaged cell membranes while showing no fluorescence in intact bacteria. The antimicrobial\u2010induced fluorescence turn\u2010on of DATVP is found to be fast (within 6 h) and sensitive, allowing for reliable determination of antimicrobial susceptibility. Using DATVP, a wash\u2010free AST is developed and its performance was validated on clinical isolates. The DATVP\u2010based AST showed high categorical agreement (84\u201095%) with the standard method while shortening the time\u2010to\u2010result from days to hours. 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