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In this study, a combination of 3D printing techniques and a modular strategy is employed to fabricate an engineered trachea, in which the decellularized extracellular matrix particles (DEPs) from diverse sources determined specific regenerative environments in different spatial regions. Costal cartilage\u2010derived DEPs are integrated within the cartilage rings of the engineered trachea. They effectively activated chondrocytes to secrete specific matrix proteins and develop into mature cartilage with a natural pattern of collagen deposition, which provided sufficient mechanical properties to maintain tracheal ventilation. Lung\u2010derived DEPsare strategically placed between the cartilage rings, and are able to accelerate endothelial cell migration to form a transmural vessel network. Additionally, lung\u2010derived DEPs exhibited a great capability to recruit macrophages and facilitate their polarization, which is beneficial for tissue regeneration. The engineered trachea underwent heterotopic vascularization and utilized for long\u2010segmental trachea replacement in a rabbit model, demonstrating a satisfactory physiological function. Through DEP functionalization, the tracheal substitute developed a native\u2010like complex structure with adequate mechanical supply, abundant blood perfusion, and favorable immune conditions, demonstrating significant clinical potential for patients requiring tracheal reconstruction.<\/jats:p>","DOI":"10.1002\/adhm.202500124","type":"journal-article","created":{"date-parts":[[2025,3,17]],"date-time":"2025-03-17T06:28:21Z","timestamp":1742192901000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["3D\u2010printed Engineered Trachea Functionalized by Diverse Extracellular Matrix Particles"],"prefix":"10.1002","volume":"14","author":[{"given":"Weikang","family":"Lin","sequence":"first","affiliation":[{"name":"Department of Thoracic Surgery Shanghai Pulmonary Hospital School of Medicine Tongji University  Shanghai 200433 China"},{"name":"Shanghai Engineering Research Center of Lung Transplantation  Shanghai 200433 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