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To enable minimally invasive delivery, a key challenge relies on making the cell sheets compatible with injection\u2010based administration without subjecting sensitive cells to physical or thermal stresses. This study addresses a reliable method for controlling cell sheet dimensions by combining differential cell adhesion\u2010guided micropatterning along with an isothermal detachment method. The surface composition of a copolymer, poly(ethylene glycol dimethacrylate\u2010co\u2010hydroxyethyl methacrylate) is delicately controlled via initiated chemical vapor deposition to ensure intact cell adhesion and rapid cell detachment under isothermal condition. The optimized surface further allows hydrophobic microcontact printing for creating micron\u2010sized sheets. Human mesenchymal stem cell sheets harvested with this method show preserved ECM without compromising cell viability after both detachment and injection. Moreover, the injected cell sheets substantially enhance the angiogenic potential of human umbilical vein endothelial cells, demonstrating the sustained therapeutic activity of the cell sheet after injection. It is believed that this approach has great potential to broaden the scope of cell sheet engineering, serving as a robust platform for regenerative medicine.<\/jats:p>","DOI":"10.1002\/adhm.202500135","type":"journal-article","created":{"date-parts":[[2025,4,18]],"date-time":"2025-04-18T08:13:31Z","timestamp":1744964011000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Reliable Harvest of Injectable Human Mesenchymal Stem Cell Sheets by Modulating Cell\u2010Substrate Adhesion Strength"],"prefix":"10.1002","volume":"14","author":[{"given":"Jemin","family":"Yeun","sequence":"first","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering Korea Advanced Institute of Science and Technology (KAIST)  291 Daehak\u2010ro, Yuseong\u2010gu Daejeon 34141 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seonghyeon","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering Korea Advanced Institute of Science and Technology (KAIST)  291 Daehak\u2010ro, Yuseong\u2010gu Daejeon 34141 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Younseong","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering Korea Advanced Institute of Science and Technology (KAIST)  291 Daehak\u2010ro, Yuseong\u2010gu Daejeon 34141 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sung Hyun","family":"Yoon","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering Korea Advanced Institute of Science and Technology (KAIST)  291 Daehak\u2010ro, Yuseong\u2010gu Daejeon 34141 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sang","family":"Yu Sun","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering Korea Advanced Institute of Science and Technology (KAIST)  291 Daehak\u2010ro, Yuseong\u2010gu Daejeon 34141 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Booseok","family":"Jeong","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering Korea Advanced Institute of Science and Technology (KAIST)  291 Daehak\u2010ro, Yuseong\u2010gu Daejeon 34141 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minkyung","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering Korea Advanced Institute of Science and Technology (KAIST)  291 Daehak\u2010ro, Yuseong\u2010gu Daejeon 34141 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyoung G.","family":"Lee","sequence":"additional","affiliation":[{"name":"Division of Nano\u2010Bio Sensors\/Chips Development National NanoFab Center (NNFC)  291 Daehak\u2010ro, Yuseong\u2010gu Daejeon 34141 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2802-6398","authenticated-orcid":false,"given":"Sung Gap","family":"Im","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering and Graduate School of Stem cell &amp; Regenerative Biology Korea Advanced Institute of Science and Technology (KAIST)  291 Daehak\u2010ro, Yuseong\u2010gu Daejeon 34141 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5542-5928","authenticated-orcid":false,"given":"Jieung","family":"Baek","sequence":"additional","affiliation":[{"name":"Department of Mechanical and Biomedical Engineering and Graduate Program in System Health Science and Engineering Ewha Woman's University  52 Ewhayeodae\u2010gil, Seodaemun\u2010gu Seoul 03760 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,4,18]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201907225"},{"key":"e_1_2_8_1_2","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.201703853"},{"key":"e_1_2_8_1_3","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0148249"},{"key":"e_1_2_8_1_4","doi-asserted-by":"publisher","DOI":"10.1016\/j.reth.2018.10.006"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2010.0470"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2021.120908"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-022-02942-9"},{"key":"e_1_2_8_5_1","volume":"2021","author":"Akiyama Y.","year":"2021","journal-title":"Cyborg. 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