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However, magnetic hyperthermia in malignant melanomas with poor immunogenicity and an immunosuppressive microenvironment remains challenging. Here, all\u2010in\u2010one magnetic nanoparticles (\u03b1PD\u2010L1\/CpG@MCL) are developed for thermo\u2010immunotherapy that enables hyperthermia with magnetic nanoparticles and immunotherapy with anti\u2010PD\u2010L1 antibody and CpG to improve the immunosuppressive tumor microenvironment. Magnetic hyperthermia with magnetite cationic liposomes (MCL, without anti\u2010PD\u2010L1 antibody and CpG) inhibits tumor growth in B16F10 melanoma; however, complete tumor regression is not observed. In contrast, complete tumor regression is observed when mice are treated with thermo\u2010immunotherapy using \u03b1PD\u2010L1\/CpG@MCL. Additionally, mice cured after magnetic hyperthermia with \u03b1PD\u2010L1\/CpG@MCL rejected rechallenge with B16F10 cells, and cytotoxicity assay using splenocytes from cured mice shows the induction of antitumor immunity against B16F10 cells. Analysis of tumor\u2010infiltrating lymphocytes reveals that magnetic hyperthermia with \u03b1PD\u2010L1\/CpG@MCL increased CD8<jats:sup>+<\/jats:sup> T lymphocytes and M1\u2010like macrophages, and decreased M2\u2010like macrophages, indicating an improvement in the tumor microenvironment. This study provides a key design strategy for all\u2010in\u2010one magnetic nanoparticles that could cure malignant melanomas.<\/jats:p>","DOI":"10.1002\/adhm.202500260","type":"journal-article","created":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T17:08:23Z","timestamp":1741712903000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["All\u2010In\u2010One Magnetic Nanoparticles for Thermo\u2010Immunotherapy of Malignant Melanoma"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8309-4086","authenticated-orcid":false,"given":"Masahiro","family":"Kaneko","sequence":"first","affiliation":[{"name":"Department of Chemical Systems Engineering Graduate School of Engineering Nagoya University  Furo\u2010cho Chikusa\u2010ku Nagoya 4648603 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rintaro","family":"Saito","sequence":"additional","affiliation":[{"name":"Department of Chemical Systems Engineering Graduate School of Engineering Nagoya University  Furo\u2010cho Chikusa\u2010ku Nagoya 4648603 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ami","family":"Nishikawa","sequence":"additional","affiliation":[{"name":"Department of Chemical Systems Engineering Graduate School of Engineering Nagoya University  Furo\u2010cho Chikusa\u2010ku Nagoya 4648603 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8393-0518","authenticated-orcid":false,"given":"Akira","family":"Ito","sequence":"additional","affiliation":[{"name":"Department of Chemical Systems Engineering Graduate School of Engineering Nagoya University  Furo\u2010cho Chikusa\u2010ku Nagoya 4648603 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3,4]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1263\/jbb.100.1"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3109\/02656736.2015.1120889"},{"key":"e_1_2_9_3_1","first-page":"305","volume":"9","author":"Han H. 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