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However, isolating tumor\u2010derived extracellular vesicles (EVs) from body fluids is often impeded by background noise, making subsequent analysis challenging. Herein, a bio\u2010inspired 3D silicon microanemone (SMA) microfluidic chip is reported. This innovative structure is prepared by a two\u2010step lithographic method combined with nanosphere lithography, achieving an impressive isolation efficiency of 89.4%. Simulation results reveal that the hierarchical structure not only provides more antibody binding sites but also synergizes with an integrated chaotic mixer to amplify fluid perturbations, while inducing a flow around circular cylinder phenomenon to enhance EV\u2010antibody interactions. Finally, the SMA chip's performance is assessed with clinical samples and combined with RT\u2010qPCR\u2010based \u03b2\u2010actin (<jats:italic>ACTB<\/jats:italic>) mRNA quantification in purified EVs. 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