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However, no commercially available GBR membrane meets all these criteria simultaneously. In this study, a novel biodegradable piezoelectric double\u2010layered membrane is developed, with a non\u2010piezoelectric Poly\u2010L\u2010lactic acid (PLLA) side facing the gingiva and a piezoelectric PLLA\u2010ZnO side facing the alveolar bone. This asymmetric GBR membrane, with distinct fiber orientations and charge distribution, combines and synergizes mechanical strength, degradability, barrier function, antibacterial activity and osteogenic potential to enhance bone regeneration efficacy. The GBR membrane can effectively prevent fibroblast migration, inhibits bacterial infection, and promotes bone regeneration both in vitro and in vivo. In vitro testing shows good antibacterial rate against <jats:italic>Porphyromonas gingivalis (P. gingivalis)<\/jats:italic>\u00a0and <jats:italic>Staphylococcus aureus (S. aureus)<\/jats:italic> after 10 min of ultrasound stimulation. Expression levels of osteogenic genes Bone morphogenetic Protein 2 (<jats:italic>BMP2<\/jats:italic>), Runt\u2010related transcription factor 2 (<jats:italic>RUNX2<\/jats:italic>), Osteopontin (<jats:italic>OPN<\/jats:italic>) and Osteocalcin (<jats:italic>OCN<\/jats:italic>) are over twice that of the control. In a mouse <jats:italic>P. gingivalis<\/jats:italic>\u2010mediated periodontitis model, our composite membrane demonstrates effective antimicrobial effects and promote bone regeneration after 2\u2010 and 4\u2010weeks implantation, facilitated by mechanisms such as physical isolation, zinc ion release, piezoelectric effects, enhanced expression of osteogenic genes through activation of osteogenesis\u2010related signaling pathways, underscoring its strong potential for GBR applications.<\/jats:p>","DOI":"10.1002\/adhm.202500543","type":"journal-article","created":{"date-parts":[[2025,3,28]],"date-time":"2025-03-28T21:15:08Z","timestamp":1743196508000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Biodegradable Piezoelectric Janus Membrane with Enhanced Antibacterial and Osteoinductive Properties for Periodontitis Therapy"],"prefix":"10.1002","volume":"14","author":[{"given":"Jingrong","family":"Cheng","sequence":"first","affiliation":[{"name":"Xiangya Stomatological Hospital and Xiangya School of Stomatology Central South University &amp; Hunan Engineering Research Center for Oral Digital Intelligence and Personalized Medicine  72 Xiangya Road Changsha Hunan 410008 P. 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