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Raman spectroscopy, an inelastic light scattering technique, reflects the biochemical building blocks (amides, sulfates, and hydroxyls) comprising a tissue. Here, an arthroscopy\u2010compatible probe acquires Raman spectra, and a multivariate linear decomposition routine extracts the regression coefficient biomarkers that reflect the contribution of extracellular matrix (ECM) constituents (sulfated glycosaminoglycans [sGAG], collagen, water) and the scaffold biomaterial to the tissue spectra. Repeated Raman acquisitions during cultivation do not alter growth of chondrocyte\u2010seeded\u2010agarose constructs. The Raman\u2010derived ECM biomarkers portray the composition of the evolving neocartilage developed on agarose, hyaluronan, collagen, and polyethylene\u2010glycol scaffolds, accounting for 90%, 78%, and 87% of content variation in sGAG, collagen, and water, and 94% of the variation in stiffness. The ECM biomarkers reveal variability in sGAG and collagen content for constructs infused with donor chondrocytes from a 58\u2010year\u2010old\/female, a 36\u2010year\u2010old\/male, and a 53\u2010year\u2010old\/male, accounting for 81% and 87% of the variation in stiffness and sGAG content. This Raman platform offers a transformative approach enabling optimization of construct fabrication, improving preclinical evaluation, and advancing cartilage regenerative therapies.<\/jats:p>","DOI":"10.1002\/adhm.202500552","type":"journal-article","created":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T04:15:24Z","timestamp":1759724124000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Evaluation of Engineered Cartilage Composition and Function Using Raman Spectroscopy"],"prefix":"10.1002","volume":"14","author":[{"given":"Dev R.","family":"Mehrotra","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering Boston University  44 Cummington Mall Boston MA 02215 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina V.","family":"Cordova","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Boston University  44 Cummington Mall Boston MA 02215 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianbai","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Materials Science and Engineering Boston University  15 St. Mary's Street Boston MA 02215 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erik E.","family":"Ersland","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering Boston University  110 Cummington Mall Boston MA 02215 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juncheng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Boston University  44 Cummington Mall Boston MA 02215 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven J.","family":"Staffa","sequence":"additional","affiliation":[{"name":"Boston Children's Hospital  300 Longwood Ave Boston MA 02115 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas P.","family":"Schaer","sequence":"additional","affiliation":[{"name":"Department of Clinical Studies, New Bolton Center, School of Veterinary Medicine University of Pennsylvania 382 W Street Rd Kennett Square PA 19348 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark W.","family":"Grinstaff","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Boston University  44 Cummington Mall Boston MA 02215 USA"},{"name":"Department of Materials Science and Engineering Boston University  15 St. Mary's Street Boston MA 02215 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian D.","family":"Snyder","sequence":"additional","affiliation":[{"name":"Center for Advanced Orthopaedic Studies Beth Israel Deaconess Medical Center 330 Brookline Ave Boston MA 02215 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mads S.","family":"Bergholt","sequence":"additional","affiliation":[{"name":"Center for Craniofacial &amp; 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